comprehensive primer design for analysis of population genetics in non-sequenced organisms全面的引物设计的群体遗传学分析不连续的生物.pdfVIP

Comprehensive Primer Design for Analysis of Population Genetics in Non-Sequenced Organisms Ayumi Tezuka, Noe Matsushima, Yoriko Nemoto, Hiroshi D. Akashi, Masakado Kawata*, Takashi Makino* Department of Ecology and Evolutionary Biology, Graduate School of Science, Tohoku University, Sendai, Japan Abstract Nuclear sequence markers are useful tool for the study of the history of populations and adaptation. However, it is not easy to obtain multiple nuclear primers for organisms with poor or no genomic sequence information. Here we used the genomes of