原创力文档determination of real-time efflux phenotypes in escherichia coli acrb binding pocket phenylalanine mutants using a 1,2′-dinaphthylamine efflux assay实时测定流出表型绑定大肠杆菌acrb口袋里使用1苯基丙氨酸突变体,2u2032-dinaphthylamine射流试验.pdfVIP
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determination of real-time efflux phenotypes in escherichia coli acrb binding pocket phenylalanine mutants using a 1,2′-dinaphthylamine efflux assay实时测定流出表型绑定大肠杆菌acrb口袋里使用1苯基丙氨酸突变体,2u2032-dinaphthylamine射流试验
Determination of Real-Time Efflux Phenotypes in
Escherichia coli AcrB Binding Pocket Phenylalanine
Mutants Using a 1,29-Dinaphthylamine Efflux Assay
¨
Jurgen A. Bohnert*, Sabine Schuster, Magdalena Szymaniak-Vits, Winfried V. Kern
Department of Medicine, Center for Infectious Diseases and Travel Medicine, Albert-Ludwigs-University, University Hospital, Freiburg, Germany
Abstract
To evaluate the importance of phenylalanine residues for substrate transport in the Escherichia coli efflux pump protein
AcrB, we subjected Phe-to-Ala binding pocket mutants to a real-time
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