development of an in vitro compartmentalization screen for high-throughput directed evolution of [fefe] hydrogenases发展高通量的体外屏幕划分(象皮病)氢化酶的定向进化.pdfVIP

Development of an In Vitro Compartmentalization Screen for High-Throughput Directed Evolution of [FeFe] Hydrogenases 1 1,2 James A. Stapleton , James R. Swartz * 1 Department of Chemical Engineering, Stanford University, Stanford, California, United States of America, 2 Department of Bioengineering, Stanford University, Stanford, California, United States of America Abstract Background: [FeFe] hydrogenase enzymes catalyze the formation and dissociation of molecular hydrogen with the help of a