原创力文档discovery of potential pirnas from next generation sequences of the sexually mature porcine testes发现潜在pirnas下一代序列性的成熟猪睾丸.pdfVIP
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discovery of potential pirnas from next generation sequences of the sexually mature porcine testes发现潜在pirnas下一代序列性的成熟猪睾丸
Discovery of Potential piRNAs from Next Generation
Sequences of the Sexually Mature Porcine Testes
1 1 1 1 1 1 2 1
Gang Liu , Bin Lei , Yan Li , Keya Tong , Yi Ding , Lifan Luo , Xuanyan Xia , Siwen Jiang ,
1 1 1
Changyan Deng , Yuanzhu Xiong , Fenge Li *
1 Key Laboratory of Pig Genetics and Breeding, Ministry of Agriculture and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of
Education, Huazhong Agricultural University, Wuhan, People’s Republic of China, 2 College of Science, Huazhong Agricultural University, Wuhan, People’s Republic of
China
Abstract
Piwi- interacting RNAs (piRNAs), a new class of small RNAs discovered from mammalian testes, are involved in transcriptional
silencing of retrotransposons and other genetic elements in germ line cells. In order to identify a full transcriptome set of
piRNAs expressed in the sexually mature porcine testes, small RNA fractions were extracted and were subjected to a Solexa
deep sequencing. We cloned 6,913,561 clean reads of Sus Scrofa small RNAs (18–30 nt) and performed functional
characterization. Sus Scrofa small RNAs showed a bimodal length distribution with two peaks at 21 nt and 29 nt. Then from
938,328 deep-sequenced small RNAs (26–30 nt), 375,195 piRNAs were identified by a k-mer scheme and 326 piRNAs were
identified by homology searches. All piRNAs predicted by the k-mer scheme were then mapped to swine genome by Short
Oligonucleotide Analysis Package (SOAP), and 81.61% of all uniquely mapping piRNAs (197,673) were located to 1124
defined genomic regions (5.85 Mb). Within these regions, 536 and 501 piRNA clusters generally distributed across
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