dnase 2 is the main dna-degrading enzyme of the stratum corneumdnase 2是主dna-degrading角质层的酶.pdfVIP

DNase 2 Is the Main DNA-Degrading Enzyme of the Stratum Corneum 1 1 1 1 2 3 Heinz Fischer , Jennifer Scherz , Sandra Szabo , Michael Mildner , Charaf Benarafa , Alicia Torriglia , 1,4 1 Erwin Tschachler , Leopold Eckhart * 1 Department of Dermatology, Medical University of Vienna, Vienna, Austria, 2 Theodor Kocher Institute, University of Bern, Bern, Switzerland, 3 INSERM UMR 872, ´ Physiopathologie des maladies oculaires, Centre de Recherches des Cordeliers, Paris, France, 4 Centre de Recherches et d’Investigations Epidermiques et Sensorielles, Neuilly-sur-Seine, France Abstract The cornified layer, the stratum corneum, of the epidermis is an efficient barrier to the passage of genetic material, i.e. nucleic acids. It contains enzymes that degrade RNA and DNA which originate from either the living part of the epidermis or from infectious agents of the environment. However, the molecular identities of these nucleases are only incompletely known at present. Here we performed biochemical and genetic experiments to determine the main DNase activity of the stratum corneum. DNA degradation assays and zymographic analyses identified the acid endonucleases L-DNase II, which is derived from serpinB1, and DNase 2 as candidate DNases of the cornified layer of the epidermis. siRNA-mediated knockdown of serpinB1 in human in vitro skin models and the investigation of mice deficient in serpinB1a demonstrated that serpinB1-derived L-DNase II is dispensable