drosophila immunity analysis of pgrp-sb1 expression, enzymatic activity and function果蝇免疫分析pgrp-sb1表达、酶活性和功能.pdfVIP

Drosophila Immunity: Analysis of PGRP-SB1 Expression, Enzymatic Activity and Function ´ 1¤ 1 ´ 2 3 3 Anna Zaidman-Remy , Mickael Poidevin , Mireille Herve , David P. Welchman , Juan C. Paredes , 4 4 2 1,3 Carina Fahlander , Hakan Steiner , Dominique Mengin-Lecreulx , Bruno Lemaitre * ´ ´ ´ ´ ´ 1 Centre de Genetique Moleculaire du CNRS, Gif-sur-Yvette, France, 2 Institut de Biochimie et Biophysique Moleculaire Cellulaire, UMR 8619 CNRS, Universite Paris-Sud, Orsay, France, 3 EPFL, Global Health Institute, Lausanne, Switzerland, 4 Department of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm, Sweden Abstract Peptidoglycan is an essential and specific component of the bacterial cell wall and therefore is an ideal recognition signature for the immune system. Peptidoglycan recognition proteins (PGRPs) are conserved from insects to mammals and able to bind PGN (non-catalytic PGRPs) and, in some cases, to efficiently degrade it (catalytic PGRPs). In Drosophila, several non-catalytic PGRPs function as selective peptidoglycan receptors upstream of the Toll and Imd pathways, the two major signalling cascades regulating the systemic production of antimicrobial peptides. Recognition PGRPs specifically activate the Toll pathway in response to Lys-type peptidoglycan found in most Gram-positive bacteria and the Imd pathway in response to D