drosophila heartless acts with heartbrokendof in muscle founder differentiation果蝇的行为与heartbrokendof肌肉创始人分化.pdfVIP

Open access, freely available online PLoS BIOLOGY Drosophila Heartless Acts with Heartbroken/Dof in Muscle Founder Differentiation Devkanya Dutta, Sanjeev Shaw, Tariq Maqbool, Hetal Pandya, K. VijayRaghavan* National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India The formation of a multi-nucleate myofibre is directed, in Drosophila, by a founder cell. In the embryo, founders are selected by Notch-mediated lateral inhibition, while during adult myogenesis this mechanism of selection does not appear to operate. We show, in the muscles of the adult abdomen, that the Fibroblast growth factor pathway mediates founder cell choice in a novel manner. We suggest that the developmental patterns of Heartbroken/Dof and Sprouty result in defining the domain and timing of activation of the Fibroblast growth factor receptor Heartless in specific myoblasts, thereby converting them into founder cells. Our results point to a way in which muscle differentiation could be initiated and define a critical developmental function for Heartbroken/Dof in myogenesis. Citation: Dutta D, Shaw S, Maqbool T, Pandya H, VijayRaghavan K (2005) Drosophila Heartless acts with Heartbroken/Dof in muscle founder differentiation. PLoS Biol 3(10): e337. Introduction mechanism [15–17]. The formation of each adult muscle fibre appears to be nucleated by a duf-lacZ-expressing cell, and these Each multi-nucleate muscle fibre in an animal is uniquely cells exhibit features characteristic of founders [15]. In adult positioned and performs a specific function. The development myogenesis, duf-lacZ expression in a founder pattern is of t