补益营卫方药对衰老人皮肤成纤维细胞数量和增殖周期的影响.docVIP

补益营卫方药对衰老人皮肤成纤维细胞数量及增殖周期的影响 何多谊,周小平1,刘韬1,徐武清1 （宁夏医科大学中医学院，银川 750004） 摘要:目的 观察经补益营卫药物血清刺激后，衰老人皮肤成纤维细胞数量和增殖周期的变化规律。方法 体外培养正常人皮肤成纤维细胞，当人皮肤成纤维细胞衰老时，加入不同浓度的药物血清配制成的细胞培养液，对衰老人皮肤成纤维细胞进行培养，通过细胞计数法连续观察细胞数量的变化；采用流式细胞术观察细胞增殖周期。结果 补益营卫药物血清高、中、低剂量组中，细胞数量和S期细胞比例均增高，且均高于对照组，但以高剂量组细胞数量和S期细胞增高比例最明显。结论 补益营卫方药促进衰老人皮肤成纤维细胞增殖,且增殖程度随着药物血清浓度的增高而相应增高。 关键词:营卫；成纤维细胞；细胞增殖；细胞周期 Influence of the recipe of supplementing Ying-wei on the proliferation and cell cycle of the aging human skin fibroblasts HE Duo-yi1, ZHOU Xiao-ping1, LIU Tao1,XU Wu-qing1 (Chinese Medicine College of Ningxia Med. Univ. , YinCHuan 750004) Abstract：Objective To observe the influence of the drug serum of supplementing Ying-wei on the proliferation and cell cycle of the aging human skin fibroblasts Methods normal human skin fibroblasts are cultured in vitro, When the normal human skin fibroblasts are decrepit,we add cell culture medium which is preparated by the drug serum with different concentrations to cultured the aging human skin fibroblasts, to observe continuously the cells?changes in the number through cell counting, we observe the cycle of cell proliferation by flow cytometry. Results Among supplementing Ying-wei drug serum with different levels of high, medium and low dose groups, the amount and the percentage of S phase cells in cell cycle of the aging human skin fibroblasts are increased significantly, and also higher than that of control group, but the high dose group are increased the most obvious. Conclusion The recipe of supplementing Ying-wei can promote the proliferation of the aging skin fibroblast, and which is increased with the increasing of drug serum concentrations of the recipe. Key words: Ying-wei; fibroblasts; cell proliferation; cell cycle 营卫具有“熏肤、充身、泽毛、温分、充皮肤、肥腠理、司开合”的作用，与皮肤生理病理有十分密切的关系。营卫的盛衰与皮肤衰老有联系Gibco公司,美国）；DMSO（Sigma公司，美国）；青霉素、链霉素（华北制药股份有限公司）；碘化丙啶(PI)（Sigma公司,美国）；流式细胞仪(BD公司,美国)；CO2孵箱(XX-50-Ⅰ型,沈阳伟明医疗设备厂)；超净工作台(SW-CJ-1F型,苏州安泰空气技术有限公司)。 1.３ 药物 补益营卫方药由人参5g、黄芪15g和