利用苯扎氯铵抑制再生水源热泵系统中微生物污垢的试验研究.pdfVIP

利用苯扎氯铵抑制再生水源热泵系统中微生物污垢的实验研究 常思远，姜朔，杨倩鹏，史琳 （清华大学热科学与动力工程教育部重点实验室，CO2 资源利用与减排技术北京市重点实验室， 清华大学热能工程系，北京，100084） 摘 要 再生水源热泵系统能够以城市再生水作为冷热源为城市建筑制冷、供暖，而微生物污垢是影响再生 水源热泵系统效率和安全的重要问题。本研究进行挂片实验生成微生物污垢，利用不同质量分数的杀菌剂苯 扎氯铵每天浸泡污垢10 min，考察其抑垢作用。对污垢进行活性染色，并利用共聚焦激光扫描显微镜CLSM 对其进行拍照，观察污垢内细胞的活性分布。最后利用Imaris 软件分别统计活/死细胞所占的生物体积。结果 表明，苯扎氯铵对微生物污垢具有抑制作用，能杀灭其中绝大部分细菌细胞，且该作用随其质量分数上升而 增强。该研究可对相关抑垢技术提供指导，从而促进再生水源热泵系统的实际应用。 关键词 微生物污垢，苯扎氯铵，激光共聚焦扫描显微镜，Imaris The Anti-fouling Effect of Benzalkonium Chloride against Biofouling in the Treated Sewage Heat Pump System CHANG Siyuan, JIANG Shuo, YANG Qianpeng, SHI Lin (Key laboratory for Thermal Science and Power Engineering of Ministry of Education, Beijing Municipal Key Laboratory for CO2 Utilization Reduction, Department of Thermal Engineering of Tsinghua University, Beijing, China, 100084) Abstract The treated sewage heat pump system reuses the wasted heat in the city sewage to refrigerate and heat the buildings. Biofouling, as the key problem in treated sewage heat pump systems, decreases the system efficiency and security. In this study, rotary coupon experiments were conducted to form biofouling on coupons. Benzalkonium chloride, a broad-spectrum and efficient bactericide, of various concentrations was used to immerse the biofouling 10 min per day. Vital dye was used to stain the biofouling and confocal laser scanning microscope (CLSM) was used to take microphotographs of the biofouling to show the viability distribution of the bacteria cells in the biofouling. Imaris was used to calculate the biovolume of live/dead cells. The results show that benzalkonium chloride inhibits the biofouling significantly by killing most of the bacteria cells, and the inhibition effect is enhanced as the concentration increases. This research