阿霉素对K562细胞Gfi-1表达影响及其及相关凋亡基因关系探究.docVIP

阿霉素对K562细胞Gfi?1表达影响及其及相关凋亡基因关系探究 　 ; 作者：常伟　孙汉英　黄敏　周剑锋 张义成 毕业论文 【摘要】; 　　本研究探讨阿霉素体外作用于K562细胞的细胞效应及癌基因Gfi?1和相关凋亡基因表达变化的机制。用不同浓度的阿霉素作用于K562细胞24小时,然后应用DNA电泳和流式细胞术检测K562细胞凋亡;用RT?PCR、流式细胞术检测Gfi?1、Bcl?2、bax 基因和蛋白表达的变化。结果表明：当阿霉素浓度在0、0.5、2.0 mg/L作用K562 细胞24小时,细胞凋亡增加,可见典型的DNA断裂电泳条带;同时,当ADM浓度自0.5增为2.0 mg/L时, Gfi?1表达减少,bax表达增加;Bcl?2表达在ADM 0.5-2.0 mg/L之间变化不明显,mRNA及蛋白水平均无统计学差异；当阿霉素浓度大于2.0 mg/L时,细胞凋亡率并不增加,而是下降,且出现细胞坏死。结论：　一定浓度阿霉素能诱导K562 细胞凋亡,细胞凋亡率表达的变化与阿霉素浓度呈一定的量效依赖关系, 阿霉素诱导K562 细胞凋亡可能与抑制Gfi?1基因的表达和激活bax 基因的表达有一定的关系。 毕业论文 【关键词】; 阿霉素　 K562 细胞； 　细胞凋亡；　Gfi?1； Bcl ? 2； 　bax 毕业论文 　　Effect of Adriamycin on; Gfi1 Expression; in K562 Cells and Its Relationship with; the Relevant Apoptotic Genes 毕业论文 ; Abstract ; The study was purposed to explore the effect of; adriamycin (ADM) on K562 cells in vitro; and the mechanism of expression changes of relevant apoptotic genes and oncogene Gfi?1.　The apoptosis was assayed by flow cytometry (FCM) and the DNA electrophoresis; the expression changes of Gfi?1、Bcl?2、bax mRNA and protein were detected; by RT?PCR and FCM after K562 cells were treated with different concentrations of ADM for 24 hours. The results showed that when K562 cells were treated with 0-2.0 mg/L ADM for 24 hours, the typical apoptotic DNA electrophoresis band of K562 cells were observed with the dose increasing. When concentration of ADM was 0.5 and 2.0 mg/L, the expression of Gfi?1 decreased and the expression of bax increased when concentration of ADM was 0.5-2.0 mg/L, the expression of Bcl?2 was not found to be significantly changed, the; levels of Bcl?2 mRNA and protein were of no statistical difference. When dose of ADM was; higher than 2.0 mg/L,; the percentage of apoptotic K562 cells decreased with cell necrosis. It is concluded; that at certain range of concentration, apoptosis or necrosis of K562 cells can; be induced by ADM, the percentage of apoptosis, the changes of expression of Bcl?2, bax and; Gfi?1 depend on the dose of ADM. The mechanism of apoptosis in