香烟烟雾提取物对气道上皮细胞增殖及FIP200表达影响.docVIP

香烟烟雾提取物对气道上皮细胞增殖及FIP200表达影响 　【关键词】 气道上皮细胞 Effect of cigarette smoke extract on proliferation and cycle of human airway epithelial cell and its FIP200 expression 　　【Abstract】 AIM: To study the effect of cigarette smoke extract （CSE ） on the proliferation and cycle of human airway epithelial cell and its mechanism. METHODS: After airway epithelial cells were treated with CSE, the proliferation was measured with MTT and the distribution of different cell cycles was detected with flow cytometry. FAKfamily interacting protein of Mr 200 000 (FIP200) mRNA expression level, FAKfamily interacting prot