IL-17A及Th17在炎症性肠病中作用.docVIP

IL-17A及Th17在炎症性肠病中作用 　 作者：杨淑萍　智绪亭　卢雪峰　冯立娟　王琴伊 【摘要】 目的：检测IL-17A、IL-10、IFN-γ在缓解期炎症性肠病（inflammatory bowl disease，IBD）患者中的表达，探讨诸炎症因子及Th17在炎症性肠病的发病及治疗中的作用。方法：应用ELISA检测12例Crohn病（Crohn disease, CD）、46例溃疡性结肠炎患者（ulcerative colitis, UC）和20例健康人（正常对照组）血清中IL-17A、IL-10和IFN-γ的水平，并用免疫组织化学法检测CD、UC及正常对照组结肠黏膜局部IL-17A的表达。结果：正常对照组结肠黏膜局部未检测到IL-17A的表达，而UC和CD组在结肠黏膜活组织中均检测到IL-17A的表达。同样UC和CD组血清中IL-17A明显增高，而正常对照组血清未检出IL-17A；UC和CD组患者血清IL-10水平均较对照组升高（Plt;0.05），且CD组明显高于UC组（Plt;0.05）；CD、UC组与正常对照组血清中IFN-γ的表达差异无统计学意义（Pgt;0.05）。结论：IL-17A不仅在缓解期IBD患者结肠黏膜局部表达，同时在其血清中高表达，提示Th17及其分泌的IL-17A在缓解期IBD患者的发病过程中起重要作用，并伴随促炎因子和抑炎性因子失调。 【关键词】 结肠炎，溃疡性·Crohn病·白细胞介素17·白细胞介素10 【ABSTRACT】 Objective: To detect the expression of IL-17A、IL-10 and IFN-γ in patients wth different forms of inflammatory bowel disease(IBD) during clinical remission phase and investigate their role in the onset and treatment of IBD. Methods: Tissue samples were obtained from patients with ulcerative colitis (UC, n=46), Crohn’s disease (CD, n=12), and normal colorectal tissues (n=20). IL-17A expression was evaluated by a standard immunohistochemical procedure. Serum IL-17A、IL-10 and IFN-γ levels were determined by ELISA. Results: IL-17A expression was not detected in samples from normal colonic mucosa but was present in the mucosa of IBD. The level of IL-17A significantly increased in IBD patients while it was not detected in the sera of normal individuals. The level of IL-10 in patients with Crohn’s disease was significantly higher than that in patients with ul cerative colitis and control group (Plt;0.05 and Plt;0.05, respectively). Conclusions: The expression of IL-17A in both the mucosa and serum of remission IBD patients increased significantly, the imbalance between production of proinflammatory and anti-inflammatory cytokines persisted even during remission of the disease. 【KEY WORDS】 Colitis, ulcerative·Crohn disease·Interleukin-17·Interleukin-10·Interferon type II 　　Thl7细胞是新近发现的一群以分泌IL-17A为特征的CD4+ T细胞亚群，具有促进免疫反应的效应，在以慢性炎症为损伤机制的自身免疫疾病