MALT1及Bcl2在MALT及DLBCL中表达及意义.doc

MALT1及Bcl2在MALT及DLBCL中表达及意义 　 作者：王松，杨文秀，刘启兰，郭宏伟，裴媛媛 【摘要】 目的： 了解MALT1和Bcl2表达对黏膜相关组织结外边缘区B细胞淋巴瘤（MALT）和弥漫大B细胞淋巴瘤（DLBCL）的影响及肿瘤中两种蛋白表达的相互关系。方法： 收集MALT和DLBCL 64例，常规HE及免疫组织化学染色，观察两种淋巴瘤的组织形态学特点，检查肿瘤细胞的免疫表型和MALT1、Bcl2的表达，收集临床病理资料并随访，对所有资料进行统计学分析。结果： MALT1和Bcl2两种蛋白表达在MALT和DLBCL差异无显著性，较晚临床分期病例的表达明显多于较早分期病例，有淋巴结累及病例的表达明显多于无累及病例，肿瘤中两种蛋白的表达有明显的相关关系，生存状况MALT1阳性病例较阴性病例差、Bcl2阳性病例较阴性病例差，但无统计学意义。结论： Bcl2在MALT和DLBCL的表达可能与MALT1引起的NFκB异常活化有关，两种蛋白的表达对MALT和DLBCL的临床病理特征有明显影响。 【关键词】 淋巴瘤，黏膜相关淋巴样组织； 黏膜相关淋巴瘤易位基因1； Bcl2 　　［Abstract］ Objective: To investigate the expression of MALT1 and Bcl2 in mucosaassociated lymphoid tissue (MALT) and diffuse large B cell lymphoma (DLBCL) as well as their effects on these lymphomas, and to understand the correlation between the expression of the two proteins in these lymphomas. Methods: Specimens of 64 cases of MALToma and DLBCL were collected. The morphology of the lymphomas were observed with HE stained sections. The immunophenotypes, such as CD20, CD3, CD5, CD10, and CyclinD1, were examined with immunohistochemistry staining method. The clinical and pathological data were collected. Some patients were followed up. Statistical analysis was done. Results: There was no statistical difference of expression between the two proteins in MALToma and DLBCL. The expressions were higher in cases with latter clinical stages than in those with earlier stages, and in cases with involvement of lymph node than in those without. There were distinct correlations between expressions of MALT1 and Bcl2 in these lymphomas. The survival status was worse in MALT1postive cases than in MALT1negative cases, and in Bcl2positive cases than in Bcl2negative cases, although the difference was not statistically significant. Conclusions: The expression of Bcl2 may associated with abnormal activation of NFκB resulted from MALT1 in MALToma and DLBCL. The expression of MALT1 and Bcl2 affects the clinicopathological features of the two lymphomas. 　　［Key words］lymphoma, mucosaassociated l