依那西普对磨屑诱导骨溶解影响实验探究.doc

依那西普对磨屑诱导骨溶解影响实验探究 　 作者：陈志荣，张亮，吴兴临，陆志东，金群华 【摘要】 ［目的］检测依那西普(Etanercept，Enbrel)对钛颗粒刺激巨噬细胞(macrophages，MΦ)分泌肿瘤坏死因子α(tumor necrosis factor-α，TNF-α)、白介素1(interleukin-1,IL-1)、白介素6(interleukin-6,IL-6)的影响，探讨依那西普防治人工关节无菌性松动的可能性。［方法］分离、培养小鼠腹腔巨噬细胞，24 h后分为5组。A组：仅为MΦ；B组：MΦ+钛颗粒；C组：MΦ+钛颗粒+依那西普(10 ng／ml)；D组：MΦ+钛颗粒+依那西普(100 ng／ml);E组：MΦ+钛颗粒+依那西普(1 000 ng／ml)。培养18 h后，用酶联免疫法(ELISA)检测细胞培养上清液中TNF-α、IL-1、IL-6的含量。［结果］B组TNF-α、IL-1、IL-6含量明显高于A、D、E组(P<0.001)，E组TNF-α、IL-1、IL-6的含量明显低于B组、C组(P<0.00１，E组和D组间无统计学意义(P>0.05)。［结论］钛颗粒可以刺激MΦ分泌大量的TNF-α、IL-1、IL-6，依那西普能够呈剂量依赖型地有效抑制钛颗粒诱导的MΦ分泌TNF-α、IL-1、IL-6，有望成为预防人工关节无菌性松动的药物。 【关键词】 依那西普； 磨屑； 骨溶解 Abstract：［Objective］To detect the effect of Etanercept on the Ti particles induced TNF-αIL-１ and IL-6 production by macrophage and to evaluate the validity of the etanercept on treatment of aseptic loosening of prosthesis.［Method］Separate and Cultivate mouse peritoneal macrophages were divide into 5 groups after 24 h. Group A was treated with MΦ alone,group B with MΦ+Ti particles,group C with MΦ+Ti particles + etanercept (10 ng/ml),group D with MΦ Ti particles + etanercept (100 ng/ml) ,group E with MΦ + Ti particles + etanercept (1000 ng/ml). After 18 h, the production of TNF-α IL-1 and IL-6 in culture supernatants was detected by ELISA.［Result］The levels of TNF-α IL-1 and IL-6 production were much higher in group B than those in group A, D, E (P<0.001). The levels of　TNF-α IL-1 and IL-6 production were much lower in group E than those in groupB, C (P<0.001).There were no significant difference in group E, D(P>0.05). ［Conclusion］Is shows the Ti particles could stimulate MΦ to excrete profuse TNF-Α IL-１ and IL-6 production, etanercept can significantly inhibited the production of TNF-α IL-1 and IL-6 secreted by Ti particles induced macrophages in a dose-dependent manner and hope to be a therapeutic candidate for the prevention of aseptic loosening. Key words：etanercept; wear debris; osteolysis 缘于假体周围骨溶解的人工关节无菌性松动是内植物失败的常见原因，由于缺乏临床干预去阻止或预防这种并发症，