基质金属蛋白酶-7及组织型金属蛋白酶抑制因子-1在膀胱移行细胞癌.docVIP

基质金属蛋白酶-7及组织型金属蛋白酶抑制因子-1在膀胱移行细胞癌 　作者：李文林，段建敏，马光旭，许崇伟，丁瑞志 【摘要】 目的 探讨基质金属蛋白酶-7（MMP-7）和金属蛋白酶组织抑制因子-1（TIMP-1）在膀胱移行细胞癌中的表达及意义。方法 应用免疫组织化学技术检测60例膀胱移行细胞癌［Ⅰ级25例，Ⅱ级15例，Ⅲ级20例；浅表性膀胱癌（Tis～T1）29例，浸润性（T2～T4）31例］和5例正常膀胱组织中MMP-7和TIMP-1的表达情况，χ2检验分析其表达与膀胱移行细胞癌分级分期的关系。结果 MMP-7和TIMP-1在正常膀胱组织中不表达，而在膀胱移行细胞癌组织表达较强。60例膀胱移行细胞癌标本中MMP-7阳性表达40例（66.7%）。MMP-7的阳性表达率与肿瘤的病理分级显著相关，而与肿瘤的临床分期无明显相关性。60例膀胱移行细胞癌标本中TIMP-1阳性表达32例（53.3%）。TIMP-1在不同的组织分化程度，不同临床分期之间均差异无统计学意义（P＞0.05）。而MMP-7/TIMP-1表达比值与肿瘤的病理分级显著相关。结论 MMP-7及MMP-7/TIMP-1比值可反映膀胱移行细胞癌的恶性程度，为膀胱移行细胞癌的预后判断提供一种新的参考指标。 【关键词】 膀胱肿瘤；癌；金属蛋白酶类； 金属蛋白酶组织抑制因子 ［Abstract］ Objective To evaluate the expression of matrix metalloproteinase-7（ MMP-7）and tissue inhibitor of matrix metalloproteinase-1（TIMP-1）in transitional cell carcinoma of bladder（TCCB）and its significance.Methods Immunohistochemistry was used to detect the expression of MMP-7 and TIMP-1 in 60 cases of BTCC（Grade Ⅰin 25 cases，Grade Ⅱ in 15，Grade Ⅲ in 20）and 5 subjects with normal bladder tissues.Of the 60 cases 29 had superficial（≤pT1）bladder cancer and 31 had invasive（≥pT2）bladder cancer.χ2 test was used to assess the relationship between expression of MMP-7，TIMP-1 and tumor grade，stage.Results The expression of MMP-7 and TIMP-1 were negative in all normal bladder tissues but high in BTCC.Of the 60 cases，40 were positive and 20 were negative for MMP-7.MMP-7 positivity was correlated with high histogical grade，but no significant difference was observed between superficial bladder cancer and invasive bladder cancer.32 were positive and 28 were negative for TIMP-1.TIMP-1 was no significant difference in different pathological grades or different clinical stages.But the ratio of MMP-7/TIMP-1 was correlated with high histogical grade.Conclusion MMP-7 and the ratio of MMP-7/TIMP-1 could reflect more accurately the malignant degree of BTCC.These results would provide a new significant marker in estimate of prognosis in patients with BTCC. ［Key words］ bladder neoplasmas;carcinoma;matrix metalloproteinases;