多因素复合造模法致肺阳虚大鼠模型探究.docVIP

多因素复合造模法致肺阳虚大鼠模型探究 　 作者：温慧萍 陈素红 吕圭源 刘建明 苏洁 【摘要】 [目的]探讨肺阳虚大鼠病理模型方法。[方法]采用烟熏(外邪犯肺)、冰水游泳(形寒劳倦)、常温游泳(劳倦)、服用他吧唑冰水溶液(免疫抑制、内饮生冷)多因素复 合造模法来制备肺阳虚证大鼠模型。观测大鼠体征，测定血液流变学指标。[结果]造模后期，大鼠出现喘鸣、呼吸急促、畏寒少动、精神萎靡、毛发凌乱无光泽、饮水量下降等症状;模型大鼠体重、背部温度、肛温、抓力呈显著性下降(Plt;0.05);低切变率下的全血粘度、全血还原粘度、红细胞刚性指数、电泳指数均显著性升高(Plt;0.05)。[结论]该造模方法能较好的与肺阳虚之临床症状相吻合，表明该方法是可行的。 【关键词】 肺阳虚;大鼠;血流变 　　Abstract：[Objective]We established a model of LungYang Deficiency.[Methods]The factors such as smoking(exogenous evil effect on lung)，swimming in ice water(cold body and exhaustion)，swimming in common water(exhaustion)，and taking the methimazole ice solution(immune suppression and inhale cold)were adopted to establish this LungYang Deficiency model.SD rats were treated for 35 days，and their physical signs(diet changes，drinking and other typical symptoms)were observed.The hemorheological parameters of the treated animals were measured.[Results]At the later period，rats in the LungYang Deficiency model showed several symptoms，such as wheeze，breathlessness，meanwhile，accompanied with dullness，drinking less water，chill.Compared with the normal group，the body weight and the back temperature of the treated rats were decreased significantly.The whole blood viscosity(WBV)and whole blood reduced viscosity(WBRV)on the low shear rate were significantly increased from the normal.So was found the rigidity finger，deformed finger and electrophoresis finger of RBC parameters.[Conclusion]This model is fit to the clinical investigation of LungYang Deficiency，resulting in that our method for model establishment is feasible. 　　Key words： lungyang deficiency;rats;blood hemorheology 　肺阳虚是由寒邪犯肺，伤及肺阳，以及脏腑功能失调或虚弱等引起的。中医肺阳虚动物模型的研究较少。本实验在参照烟熏结合寒冷刺激建立模型[12]的方法之上予以改进，我们前期实验采用烟熏(外邪犯肺)、冰水游泳(形寒劳倦)、服用他吧唑冰水溶液(免疫抑制、内饮生冷)等复合因素造模，四个模型组小鼠均出现体重增长缓慢，自主活动减少，抓力降低，心、肺指数增加。其中三因素组合对体征以及肺组织和血清MDA、SOD的影响，优于二因素组合;增加冰泳优于服用冰水，抓力下降、心肺指数升高更明显。为进一步观察肺阳虚模型在大鼠上的重现性，我们采用烟熏+冰泳+服他吧唑冰水溶液复合因素造模，观察其对相关指标的影响。 　　1 实验材料 　　1.1 材料 柠檬酸钠(分析纯，上海化学试剂公司、甲巯咪唑薄膜衣片(他吧唑)，规格10mg×50，批号5728