大豆异黄酮及羧甲基纤维素钠对去卵巢大鼠体重、血脂影响.docVIP

大豆异黄酮及羧甲基纤维素钠对去卵巢大鼠体重、血脂影响 　 作者：张云波 那晓琳 赵新宇 李丽娜 【摘要】 目的 研究大豆异黄酮及羧甲基纤维素钠对去卵巢大鼠肥胖的预防作用以及对血脂的改善作用。方法 清洁级7 w龄SD雌性大鼠88只，随机分为两组，喂饲不同剂量的羧甲基纤维素钠。每组内部再根据体重随机分为6亚组，1亚组为单纯开腹术组,其他5组在无菌条件下切除双侧卵巢,喂饲不同剂量的大豆异黄酮。手术后给与高脂饲料诱导肥胖，实验组同时进行受试物灌胃。结果 大豆异黄酮能显著降低去卵巢大鼠的体重、改善大鼠血脂水平，降低血清中总胆固醇(TC)、甘油三酯(TG)水平，升高高密度脂蛋白胆固醇(HDLC)水平。羧甲基纤维素钠能显著降低大鼠体重，并与大豆异黄酮具有协同作用，但其对血脂的作用甚微。结论 大豆异黄酮能够预防去卵巢大鼠的肥胖，并且改善血脂;羧甲基纤维素钠可显著减缓大鼠体重的增加，但对血脂水平影响甚微。 【关键词】 大豆异黄酮;羧甲基纤维素钠;肥胖;血脂 　　【Abstract】 Objective To investigate the beneficial effects of isoflavone and CMCNa on obesity and blood lipids in ovariectomized rats. Methods Female 8weekold SD rats were randomly divided into 2 groups and fed with different doses of CMCNa. Each group was randomly divided into 6 groups according to body weight. There were sham control group (SHAM), ovariectomized control group (OVX), OVX with lowdose isoflavone (LSI), middledose isoflavone (MSI) and highdose isoflavone (HSI), and estrogen(EC) groups. All animals were allowed free access to high fat diet and water, and administrated with isoflavone, and then sacrificed 4 weeks later. Results Compared with OVX control group, rats′ body weight were reduced in isoflavone groups. Serum TG, TC levels were reduced and HDLC levels were increased in isoflavone groups. Body weight was reduced in high CMCNa group. CMCNa had a synergism effect with isoflavone. But there was no significant effect on blood lipids. Conclusions Isoflavone can reduce the body weight induced by OVX and improve blood lipids. CMCNa can significantly reduce body weight, but there is little effect on blood lipids. 　　【Key words】 Isoflavone; CMCNa; Obesity; Blood lipids 　　 肥胖是一个严重的世界性问题，由于老年人肥胖的比例上升，使一些相关疾病如糖尿病、心血管疾病、癌症的发生率和病死率升高。我国部分城市老年人群中女性超重率为37.7%〔1〕，预防和治疗老年肥胖已成为热点问题。大豆异黄酮是一种植物雌激素，研究表明其具有明显的降低肥胖大鼠体重、改善高血脂大鼠血脂等作用〔2,3〕;羧甲基纤维素钠(CMCNa)可作为膳食纤维素的替代物，可以促进脂肪排出体外，具有减肥功效。然而大豆异黄酮及CMCNa对肥胖的预防作用以及他们之间的联合作用却鲜有报道。本研究在观察大豆异黄酮及CMCNa对去卵巢大鼠预防肥胖作用的同时，还观察了其对血脂的改善作用,为临床实践提供一点依据。 　　1 材料与方法 　　1.1 试剂 大豆异黄酮购自山东长润生物有限公司，总含量为40%。CMCNa购自宜