左旋卡尼汀预处理对离体兔心缺血-再灌注致心肌损伤及MDA、SOD.docVIP

左旋卡尼汀预处理对离体兔心缺血/再灌注致心肌损伤及MDA、SOD 　 作者：赵宁军，许铁，叶英，燕宪亮，梁高永，刘蓓 【摘要】 目的 观察左旋卡尼汀（L-CN）预处理对离体兔心缺血/再灌注致心肌损伤及丙二醛（MDA）、超氧化物歧化酶（SOD）的影响。方法 采用离体兔心Langendorff灌注实验模型,离体兔心12只随机分成缺血/再灌注组（I/R组）和L-CN预处理组，每组6只。I/R组灌注K-H液25 min，（兔心）4℃标准St.Thomas停搏液(K+ 16 mmol/L)至心脏停搏,45 min后恢复K-H液灌注20 min; L-CN预处理组灌注K-H液10 min，再予L-CN续灌15 min，余步骤同I/R组。测定再灌注末心肌组织中MDA、SOD的活性； 2，3，5-三苯基氯化四氮唑（TTC）染色测定心肌存活面积百分比。结果 L-CN预处理组SOD活性明显高于I/R组（P＜0.01），MDA含量明显低于I/R组（P＜0.05）。L-CN预处理组心肌存活面积百分比高于I/R组(P＜0.05）。结论 L-CN预处理对高钾停搏离体兔缺血/再灌注心脏具有抑制氧化应激、提高抗氧化能力的作用，可减轻心肌损伤。 【关键词】 缺血/再灌注损伤;离体心脏;左旋卡尼汀;预处理;家兔 　　Abstract: Objective To investigate the effects of L-carnitine (L-CN) preconditioning on levels of superoxide dismutase (SOD) and malondialdehyde (MDA) against ischemia/reperfusion-induced myocardial injury in isolated rabbit hearts. Methods The experiment was performed on isolated rabbit hearts perfused on Langendorff apparatus. 12 rabbit hearts were randomized into ischemia/reperfusion group (I/R group) and L-CN preconditioning group (n=6 each). The I/R group was firstly perfused with Krebs-Henseleit buffer (K-H) solution at 4℃ for 25 min, followed by St. Thomas Ⅱ solutions (K+ 16 mmol/L) until cardiac arrest for 45 min and then reperfused with K-H solution for 20 min. The treatment of L-CN preconditioning group was similar to that of the I/R group, except that this group was initially perfused with K-H solution for 10 min and then perfused with L-CN. At the end of reperfusion, the contents of MDA, the activity of SOD were measured. The percentage of survival areas of myocardium was measured by using 2,3,5-triphenyltetrazolium chloride (TTC) stain. Results In contrast to the findings in the I/R group, the percentages of surviving myocardium areas in the L-CN preconditioning group were higher (Plt;0.05). SOD content in L-CN preconditioning group was significant higher than that in I/R group (Plt;0.01). MDA content in L-CN preconditioning group was significantly lower than that in I/R group (Plt;0.05). Conclusion L-CN precondit