恒磁场对金属离子钴铬作用下人单核细胞分泌肿瘤坏死因子影响.docVIP

恒磁场对金属离子钴铬作用下人单核细胞分泌肿瘤坏死因子影响 　 作者：戴闽，熊建卫，詹 平，漆启华，郝亮 【摘要】 [目的]探讨恒磁场对金属离子钴、铬作用下人外周血单核细胞分泌肿瘤坏死因子的影响，寻找防治假体周围骨溶解的方法。[方法]将CoCl2粉末与CrCl3粉末溶于无菌注射用水配制成CoCl2溶液和CrCl3溶液，从健康人外周血提取单核细胞，将金属离子Co2+、Cr3+分别与人单核细胞在不同的磁场强度(1 mT、10 mT、100 mT)下共培养。实验分9组：单核细胞组(对照组)、Co2+、Cr3+组、Co2+、Cr3+分别+不同磁场强度(1 mT、10 mT、100 mT)组，各组细胞于培养12、24、48 h取细胞上清液，用ELISA法检测上清液中肿瘤坏死因子(TNF-α)的含量，以吸光度(A值)的高低来反映标本中肿瘤坏死因子α含量的多少。[结果]Co2+、Cr3+均能刺激人单核细胞释放TNF-α(Plt; 0. 05)，且以24 h释放最为明显。经磁场暴露12 h之后，1 mT、10 mT、100 mT磁场处理组TNF-α量均低于非磁场处理组(Plt; 0. 05)，并呈现强度依赖性。[结论]一定强度的恒磁场可拮抗金属离子钴、铬刺激人外周血单核细胞分泌TNF-α，为磁场疗法防治假体周围骨溶解提供了理论依据。 【关键词】 恒磁场; 肿瘤坏死因子; 金属离子; 无菌性松动 　　Abstract: [Objective]To detect the effect of constant magnetic field on metal ion Co2+,Cr3+ induced TNF-α secreted by human mononuclear cells, and to search a method for prevention and treatment of aseptic loosening. [Methods]CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water. Mononuclear cells from human peripheral blood, were taken and cultured with Co2+,Cr3+ ions in different magnetic field of 10Gs,100 Gs,1000Gs for 12,24 and 48 hs. There were nine groups: control group,Co2+ group,Cr3+ group,Co2+andCr3+ with various intensities of constant magnetic field,respectively.ELISA method was applied to detect tumor necrosis factor (TNF-α) in serum via the absorbance (A).[Results]Co2+ and Cr3+ ions stimulated human mononuclear cell to secrete TNF-α (Plt;0. 05). TNF-α content was obviously higher at 24 h.After the magnetic exposure time extended to 12 h, TNF-α value obviously decreased (Plt;0. 05) as compared with non-magnetic group,and it was positively related to the Aintensities of constant magnetic field.[Conclusion]Within a certain range of magnetic intensity , constant magnetic fields inhibit human mononuclear cell stimulated by Co2+andCr3+ ions to secrete TNF-α.It provides a theoretical basis for the prevention and treatment of aseptic loosening. 　　Key words：constant magnetic field; tumor necrosis factor; metal ion; aseptic loosening 　　人工关节置换术是治疗骨关节疾患的