甘薯抗薯瘟病的苯丙烷类代谢研究.PDFVIP

2009 9 17 5 Chinese Journal of Eco-Agriculture, Sept. 2009, 17(5): 944−948 DOI: 10. 3724/SP.J.1011.2009.00944 * 王 伟 1 阮妙鸿 2 邱永祥 1 王伟英 1 柯玉琴 1** 潘廷国 1 (1. 350002; 2. 350003) 甘薯瘟病是甘薯的重要病害, 严重影响甘薯的品质和产量。本试验在甘薯苗期, 通过剪叶法接菌研究 甘薯抗感病品种苯丙烷类代谢的变化规律。结果表明: 甘薯抗感病品种接菌后PAL 、POD 活性均有所提高, 且 提高速度与品种抗性呈正相关; 抗病品种接菌后木质素和绿原酸含量显著增加, 感病品种接菌后绿原酸、总酚 和类黄酮含量波动较大, 木质素含量降低。相关性分析表明, 接菌后木质素和绿原酸的积累与甘薯品种抗病性 密切相关。接菌后PAL 、POD 活性迅速提高并积累木质素和绿原酸等抗病物质是甘薯抗薯瘟病的苯丙烷类代 谢基础, 在抗病品种鉴定方面有一定的参考价值。 甘薯瘟病 苯丙烷类代谢 PAL POD 木质素 绿原酸 : S435.311 : A : 1671-3990(2009)05-0944-05 Phenylaprapanoid metabolism of sweet potato against Pseudomonas solanacearum 1 2 1 1 1 1 WANG Wei , RUAN Miao-Hong , QIU Yong-Xiang , WANG Wei-Ying , KE Yu-Qin , PAN Ting-Guo (1. College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 350002, China; 2. Fujian Agriculture Eco-environment and Energy Station, Fuzhou 350003, China) Abstract Pseudomonas solanacearum is a major diseases in sweet potato which leads to substantive yield loss and quality degra- dation. Changes in phenylaprapanoid metabolism were studied in resistant and susceptible cultivars of sweet potato seedlings after inoculation by leaf-cut. The results indicate that PAL and POD activities in resistant and susceptible cultivars increase after inocula- tion. However, the rates of increase in PAL and POD activities are directly related with cultivar resistance to pathogens. Lignin and chlorogenic acid contents increase significantly after inoculation of resistant cultivars. Though chlorogenic acid, total phenolics and flavonoids contents