没食子酰氧化芍药苷在皮肤角质化细胞中地抗氧化应激作用.pdfVIP

Original Article Biomol Ther 21(5), 349-357 (2013) 6-O-Galloylpaeoniflorin Protects Human Keratinocytes Against Oxidative Stress-Induced Cell Damage Cheng Wen Yao, Mei Jing Piao, Ki Cheon Kim, Jian Zheng, Ji Won Cha and Jin Won Hyun* School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 690-756, Republic of Korea Abstract 6-O-galloylpaeonifl orin (GPF) is a galloylated derivate of paeonifl orin and a key chemical constituent of the peony root, a peren- nial fl owering plant that is widely used as an herbal medicine in East Asia. This study is the fi rst investigation of the cytoprotective effects of GPF against hydrogen peroxide (H O )-induced cell injury and death in human HaCaT keratinocytes. GPF demonstrated 2 2 a signifi cant scavenging capacity against the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, H O -generated intracellular re- 2 2 active oxygen species (ROS), the superoxide anion radical (O2-), and the hydroxyl radical (•OH). GPF also safeguarded HaCaT keratinocytes against H O -provoked apoptotic cell death and attenuated oxidative macromolecular damage to DNA, lipids, and 2 2 proteins. The compound exerted its cytoprotective actions in keratinocytes at least in part by decreasing the number of DNA strand breaks, the levels of 8-isoprostane (a stable end-product of lipid peroxidation), and the formation of carbonylated protein species. Taken together, these results indicate that GPF may be developed as a cytoprotector