寻找同工酶酶谱鉴别指标.DOC

阴道毛滴虫和口腔毛滴虫5种同工酶谱对比分析 梁裕芬1，韦俊彬1，运晨霞2，黄庶识3 （530001 南宁，广西中医学院：寄生虫学教研室1，微生物和免疫学教研室2；530003 南宁，广西科学院生物物理实验室3） [摘要] 目的　分析阴道毛滴虫(Trichomonas vaginalis，Tv)和口腔毛滴虫(Trichomonas tenax，TT)的同工谱特征及其亲缘关系，寻找同工酶谱鉴别指标。方法　采用聚丙烯酰胺凝胶垂直板电泳，分别进行苹果酸脱氢酶(MDH)、谷氨酸脱氢酶(GLDH)、6-磷酸-葡萄糖脱氢酶（G6PD）、乙醇脱氢酶(ADH)和过氧化氢酶（Catalase）5种同工酶染色，比较分析2种滴虫的酶谱。结果 MDH同工酶：2种滴虫只有相对迁移率(Rm) 52、85 2条区带相同，而Rm 34、42、94、100 4条区带在2种滴虫之间明显不同；GLDH同工酶：TV无显带，TT共分离出6条酶带；G6PD同工酶：TV只有Rm35、42、52 3条区带，而TT除了显示这3条区带外，还显示出其他迁移率更大的区带；ADH和Catalase均无区带显示。结论　MDH、GLDH和G6PD3种同工酶均具有鉴别意义。 [关键词] 阴道毛滴虫；口腔毛滴虫；同工酶；分类 [中图法分类号] [文献标志码] A Five isozymograms of Trichomonas vaginalis and Trichomonas tenax: A comparative study Liang Yufen1, Wei Junbin1, Yun Chenxia2, Huang Shushi3 （1 Teaching and Research Section of Parasitology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China; 2Teaching and Research Section of Microbiology and Immunology, Guangxi University of Traditional Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China; 3Laboratory of Biophysics, Guangxi Academy of Sciences, Nanning 530003, Guangxi Zhuang Autonomous Region, China） [Abstract] Objective To analyze the characteristics of isozymograms of Trichomonas vaginalis (TV) and Trichomonas tenax (TT) and their relation in an attempt to find the identification index of their isozymograms. Methods Five isozymes including melate dehydrogenase (MDH), glutamic dehydrogenase (GLDH), glucose-6-phosphate dehydrogenase (G6PD), alcohol dehydrogenase (ADH) and catalase were stained with SDS. Isozymograms of TV and TT were compared and analyzed. Results Two identical bands (Rm52 and Rm85) of MDH isozymogram and 4 bands (Rm34, Rm42, Rm94 and Rm100) of MDH isozymogram were found in TV and TT, respectively. No band and 6 band of GLDH isozymogram were identified in TV and TT, respectively. Three bands (Rm35, Rm42 and Rm52) of G6PD isozymogram were observed in TV, and more bands of G6PD isozymogram were dem