参芪复方对GK大鼠大血管病变血管紧张素Ⅱ及其受体影响实验的研究.pdfVIP

成都中医药大学2007 级博士论文 ABSTRACT Objective: To elvaluate the therapeutic effect of ShenQi compound recipe(SQCR) on the diabetes mellitus macrovasculopathy GK rat,which has the function of tonifying Qi and Yin and promoting blood circulation to dissipate stasis,in the level of gene, molecular and cell leve.To demonstrate the mechanism of SQCR for diabetes mellitus macrovasculopathy from the view of angiotensin and its recepors. Methods: 73 GK male rats were divided into four groups randomly: respectively 18 in GK group, drenched normal saline 5ml ·kg-1 ·d-1, 19 in model group, drenched normal saline 5ml ·kg-1 ·d-1, 18 in atorvastatin groups, drenched atorvastatin calcium suspension 1.6mg ·kg-1 ·d-1, 18 in SQCR group, drenched SQCR suspl 1.44g ·kg-1 ·d-1. 18 SPF Wistar rats in the control group was drenched normal saline 5ml ·kg-1 ·d-1. In order to build the diabetes mellitus macroangiopathy model, the model, atorvatatin, SQCR groups were administered with the N ω-nitro-L-arginine methyl ester(L-NAME) 0.1mg ·ml-1 ·d-1 in the drinking water for 35 days. After sacrifice, detect the blood glucose, blood fat, blood secum C reactive protein (CRP) and Angiotensin Ⅱ;dectect the angiotensin Ⅱ type 1 receptor(AT1R)mRNA expression in the abdominal aorta vessel wall by real-time PCR; dectect the integral optical density of AT1R and AT2R in the abdominal aorta vessel wall by immunohistochemistry;detect the intercellular adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) by western blot. Result: The typical atheromatosis pathoalteration was observed in model group