the ratio and concentration of two monoterpenes mediate fecundity of the pinewood nematode and growth of its associated fungi两个单萜的浓度比和调解松木线虫繁殖力和增长相关的真菌.pdfVIP

The Ratio and Concentration of Two Monoterpenes Mediate Fecundity of the Pinewood Nematode and Growth of Its Associated Fungi 1,2 1 1 1 1 Hongtao Niu , Lilin Zhao , Min Lu , Shuai Zhang , Jianghua Sun * 1 State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China, 2 Graduate University of Chinese Academy of Sciences, Beijing, China Abstract The pinewood nematode (PWN) Bursaphelenchus xylophilus, vectored primarily by the sawyer beetle, Monochamus alternatus, is an important invasive pest and causal agent of pine wilt disease of Chinese Masson pine, Pinus massoniana. Previous work demonstrated that the ratios and concentrations of a-pinene:b-pinene differed between healthy trees and those trees containing blue-stain fungus (and M. alternatus pupae). However, the potential influence of the altered monoterpene ratios and concentrations on PWN and associated fungi remained unknown. Our current results show that low concentrations of the monoterpenes within petri dishes reduced PWN propagation, whereas the highest concentration of the monoterpenes increased PWN propagation. The propagation rate of PWN treated with the monoterpene ratio representative of blue-stain infected pine (a-pinene:b-pinene = 1:0.8, 137.6 mg/ml) was significantly higher than that (a- pinene:b-pinene = 1:0.1, 137.6 mg/ml) representative of healthy pines or those damaged by M. alternatus feeding, but without blue stain. Furthermore, inhibition of mycelial growth of associated fungi increased with the concentration of the monoterpenes a-pinene and b-pinene. Additionally, higher levels of b-pinene (a-pinene:b-pinene = 1:0.8) r