原创力文档the ratio 16601690 cm？1 measured by infrared microspectroscopy is not specific of enzymatic collagen cross-links in bone tissue比16601690厘米 1红外显微镜测得不是特定酶胶原交叉连接的骨组织.pdfVIP
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the ratio 16601690 cm？1 measured by infrared microspectroscopy is not specific of enzymatic collagen cross-links in bone tissue比16601690厘米 1红外显微镜测得不是特定酶胶原交叉连接的骨组织
The Ratio 1660/1690 cm 21 Measured by Infrared
Microspectroscopy Is Not Specific of Enzymatic Collagen
Cross-Links in Bone Tissue
1,2 2,3,4 1,2 1,2 ´
Delphine Farlay *, Marie-Eve Duclos , Evelyne Gineyts , Cindy Bertholon , Stephanie Viguet-
1,2 3 3 4 2,5
Carrin , Jayakrupakar Nallala , Ganesh D. Sockalingum , Dominique Bertrand , Thierry Roger ,
Daniel J. Hartmann2,6, Roland Chapurlat1,2, Georges Boivin1,2
´ ´ ´
1 INSERM, UMR 1033, Lyon, France, 2 Universite de Lyon, Lyon, France, 3 MeDIAN, Universite de Reims Champagne-Ardenne, CNRS UMR6237-MEDyC, Reims, France,
´
4 INRA, Nantes, France, 5 UPSP nu 2011-03-101, VetAgro Sup Lyon, Lyon, France, 6 UMR-CNRS 5510, Universite Claude Bernard, Lyon, France
Abstract
In postmenopausal osteoporosis, an impairment in enzymatic cross-links (ECL) occurs, leading in part to a decline in bone
biomechanical properties. Biochemical methods by high performance liquid chromatography (HPLC) are currently used to
measure ECL. Another method has been proposed, by Fourier Transform InfraRed Imaging (FTIRI), to measure a mature
PYD/immature DHLNL cross-links ratio, using the 1660/1690 cm21 area ratio in the amide I band. However, in bone, the
amide I band composition is complex (collagens, non-collagenous proteins, water vibrations) and the 1660/1690 cm21 by
FTIRI has never been directly correlated wi
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