the reconstruction of condition-specific transcriptional modules provides new insights in the evolution of yeast ap-1 proteinscondition-specific转录模块的重建提供了新的见解酵母ap-1进化的蛋白质.pdfVIP

The Reconstruction of Condition-Specific Transcriptional Modules Provides New Insights in the Evolution of Yeast AP-1 Proteins 1,2,3 1,2,3 ´ ´ 4. ¨ 1,2,3 . Christel Goudot , Catherine Etchebest , Frederic Devaux , Gaelle Lelandais * ´ ´ ´ 1 Dynamique des Structures et Interactions des Macromolecules Biologiques (DSIMB), INSERM, U665, Paris, France, 2 Universite Paris Diderot, Sorbonne Paris Cite, UMR- ´ ´ S665, Paris, France, 3 INTS, Paris, France, 4 Laboratoire de Genomique des Microorganismes, UMR7238 CNRS, Universite Pierre et Marie Curie, Paris, France Abstract AP-1 proteins are transcription factors (TFs) that belong to the basic leucine zipper family, one of the largest families of TFs in eukaryotic cells. Despite high homology between their DNA binding domains, these proteins are able to recognize diverse DNA motifs. In yeasts, these motifs are referred as YRE (Yap Response Element) and are either seven (YRE-Overlap) or eight (YRE-Adjacent) base pair long. It has been proposed that the AP-1 DNA binding motif preference relies on a single change in the amino acid sequence of the yeast AP-1 TFs (an arginine in the YRE-O binding factors being replaced by a lysine in the YRE-A binding Yaps). We developed a computational approach to infer condition-specific transcriptional modules associated to the orthologous AP-1 protein Yap1p, Cgap1p and Cap1p, in three yeast species: the model yeast Saccharomyces cerevisiae and two pathogen