the ph dependence of polymerization and bundling by the essential bacterial cytoskeltal protein ftsz聚合和绑定的ph值依赖细菌cytoskeltal蛋白质ftsz至关重要.pdfVIP

The pH Dependence of Polymerization and Bundling by the Essential Bacterial Cytoskeltal Protein FtsZ ´ ´ 1 2 4 1,3 Raul Pacheco-Gomez , David I. Roper , Timothy R. Dafforn , Alison Rodger * 1 Molecular Organization and Assembly in Cells Doctoral Training Centre, University of Warwick, Coventry, United Kingdom, 2 School of Life Sciences, University of Warwick, Coventry, United Kingdom, 3 Department of Chemistry, University of Warwick, Coventry, United Kingdom, 4 Department of Biosciences, University of Birmingham, Edgebaston, Birmingham, United Kingdom Abstract There is a growing body of evidence that bacterial cell division is an intricate coordinated process of comparable complexity to that seen in eukaryotic cells. The dynamic assembly of Escherichia coli FtsZ in the presence of GTP is fundamental to its activity. FtsZ polymerization is a very attractive target for novel antibiotics given its fundamental and universal function. In this study our aim was to understand further the GTP-dependent FtsZ polymerization mechanism and our main focus is on the pH dependence of its behaviour. A key feature of this work is the use of linear dichroism (LD) to follow the polymerization of FtsZ monomers into polymeric structures. LD is the differential absorption of light polarized parallel and perpendicular to an orientation direction (in this case that provided by shear flow). It thus readily distinguishes between FtsZ polymers and monomers. It also distinguishes FtsZ polymers and less well-defined aggregates, which light scattering methodologies do not. The polymerization of FtsZ over a range of pHs was studied by right-angled light scattering to probe mass of FtsZ structur