the peritoneum as a natural scaffold for vascular regeneration腹膜作为天然支架血管再生.pdfVIP

The Peritoneum as a Natural Scaffold for Vascular Regeneration 1 2,3 1 4 1 Stefano Bonvini *, Mattia Albiero , Luca Ferretto , Annalisa Angelini , Piero Battocchio , Marny 4 1 4 2,3 2,3 . 1 . Fedrigo , Michele Piazza , Gaetano Thiene , Angelo Avogaro , Gian Paolo Fadini * , Franco Grego * 1Vascular and Endovascular Surgery, Department of Cardiac, Thoracic and Vascular Sciences., University of Padova, Padova, Italy, 2 Department of Medicine, University of Padova, Padova, Italy, 3 Laboratory of Experimental Diabetology, Venetian Institute of Molecular Medicine, Padova, Italy, 4 Cardiovascular Pathology, Department of Cardiac, Thoracic and Vascular Sciences, University of Padova, Padova, Italy Abstract Objective: The peritoneum has the same developmental origin as blood vessels, is highly reactive and poorly thrombogenic. We hypothesize that parietal peritoneum can sustain development and regeneration of new vessels. Methods and Results: The study comprised two experimental approaches. First, to test surgical feasibility and efficacy of the peritoneal vascular autograft, we set up an autologous transplantation procedure in pigs, where a tubularized parietal peritoneal graft was covered with a metal mesh and anastomosed end-to-end in the infrarenal aorta. Second, to dissect the contribution of graft vs host cells to the newly developed vessel wall, we performed human-to-rat peritoneal patch grafting in the abdominal aorta and examined the origin of endothelial and smooth muscle cells. In p