a standard addition method to assay the concentration of biologically interesting polyphenols in grape berries by reversed-phase hplc一个标准添加法测定的浓度生物有趣的多酚类物质在葡萄浆果反相高效液相色谱法.pdfVIP
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a standard addition method to assay the concentration of biologically interesting polyphenols in grape berries by reversed-phase hplc一个标准添加法测定的浓度生物有趣的多酚类物质在葡萄浆果反相高效液相色谱法
Molecules 2007, 12, 2259-2269
molecules
ISSN 1420-3049
© 2007 by MDPI
/molecules
Full Paper
A Standard Addition Method to Assay the Concentration of
Biologically Interesting Polyphenols in Grape Berries by
Reversed-Phase HPLC
Aphrodite-Victoria Sakkiadi, Constantinos A. Georgiou and Serkos A. Haroutounian*
Chemistry Laboratory, Agricultural University of Athens, Iera odos 75, Athens 11855, Greece
* Author to whom correspondence should be addressed; E-mail: sehar@aua.gr
Received: 19 September 2007; in revised form: 26 September 2007 / Accepted: 26 September 2007 /
Published: 27 September 2007
Abstract: A reversed-phase HPLC method which allows the simultaneous assay of (+)-
catechin, (–)-epicatechin, trans-resveratrol, quercetin and quercetin glycosides in grape
berries is described. Kromasil 100 served as stationary phase and a gradient of acetic acid,
water and methanol was used. The analytical run requires 42 min for complete sample
elution. Satisfactory peak resolution was achieved following a novel extraction process and
direct injection of a 20 μL sample. The method was used for the analyses of eighteen
samples. Linearities were in the range of 0.98 to 0.999 regression coefficient, for all
phenolics, while detection limits ranged from 30 μg mL–1 for trans-resveratrol to 1.5 mg
–1
mL for (+)-catechin. Recoveries ranged from 95.1 to 98.7% while the method provided
good precision, with standard devi
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