the redox behaviour of randomly dispersed single walled carbon nanotubes both in the absence and in the presence of adsorbed glucose oxidase随机分散单壁碳纳米管的氧化还原行为在没有和吸附葡萄糖氧化酶的存在.pdfVIP

Sensors 2006, 6, 1791-1826 sensors ISSN 1424-8220 © 2006 by MDPI /sensors Full Paper The Redox Behaviour of Randomly Dispersed Single Walled Carbon Nanotubes both in the Absence and in the Presence of Adsorbed Glucose Oxidase Michael E. G. Lyons 1,* and Gareth P. Keeley 1 Physical and Materials Electrochemistry Laboratory, School of Chemistry, University of Dublin, Trinity College, Dublin 2, Ireland * Author to whom correspondence should be addressed; E-mail: melyons@tcd.ie Received: 15 November 2006 / Accepted: 18 December 2006 / Published: 20 December 2006 Abstract: The electrochemical behaviour of SWCNTs randomly dispersed on gold and glassy carbon electrode surfaces was characterised via cyclic voltammetry and complex impedance spectroscopy, using the ferri/ferrocyanide couple as a redox active test probe . In subsequent investigations glucose oxidase (GOx) was adsorbed onto the SWCNT ensemble without apparent denaturation of the enzyme. Cyclic voltammetry and potential step chronoamperometry were used to quantify and understand the process of electron transfer between the immobilised protein redox site and the working electrode. The effect of pH on the system was also investigated. In particular, we have shown that, for the calculation of electron transfer rate constants for surface- immobilised redox systems, chronoamperometry is preferable to voltammetry, which has been the technique of choice until now. Keywo