原创力文档the red fluorescent protein eqfp611 application in subcellular localization studies in higher plants红色荧光蛋白eqfp611在高等植物亚细胞定位研究中的应用.pdfVIP
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the red fluorescent protein eqfp611 application in subcellular localization studies in higher plants红色荧光蛋白eqfp611在高等植物亚细胞定位研究中的应用
BMC Plant Biology BioMed Central
Research article Open Access
The red fluorescent protein eqFP611: application in subcellular
localization studies in higher plants
Joachim Forner and Stefan Binder*
Address: Molekulare Botanik, Universität Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany
Email: Joachim Forner - joachim.forner@uni-ulm.de; Stefan Binder* - stefan.binder@uni-ulm.de
* Corresponding author
Published: 6 June 2007 Received: 8 November 2006
Accepted: 6 June 2007
BMC Plant Biology 2007, 7:28 doi:10.1186/1471-2229-7-28
This article is available from: /1471-2229/7/28
© 2007 Forner and Binder; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background: Intrinsically fluorescent proteins have revolutionized studies in molecular cell
biology. The parallel application of these proteins in dual- or multilabeling experiments such as
subcellular localization studies requires non-overlapping emission spectra for unambiguous
detection of each label. In the red spectral range, almost exclusively DsRed and derivatives thereof
are used today. To test the suitability of the red fluorescent protein eqFP611 as an alternative in
higher plants, the behavior of this protein was analyzed in terms of expression,
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