the physical interaction of myoblasts with the microenvironment during remodeling of the cytoarchitecture成肌细胞的微环境的物理相互作用在细胞结构的重构.pdfVIP

The Physical Interaction of Myoblasts with the Microenvironment during Remodeling of the Cytoarchitecture 1 1 1 1 Daniel J. Modulevsky , Dominique Tremblay , Corinne Gullekson , Nickolay V. Bukoresthliev , Andrew E. Pelling1,2,3* 1 Department of Physics, Centre for Interdisciplinary NanoPhysics, University of Ottawa, Ottawa, Ontario, Canada, 2 Department of Biology, University of Ottawa, Ottawa, Ontario, Canada, 3 Institute for Science Society and Policy, University of Ottawa, Ottawa, Ontario, Canada Abstract Integrins, focal adhesions, the cytoskeleton and the extracellular matrix, form a structural continuum between the external and internal environment of the cell and mediate the pathways associated with cellular mechanosensitivity and mechanotransduction. This continuum is important for the onset of muscle tissue generation, as muscle precursor cells (myoblasts) require a mechanical stimulus to initiate myogenesis. The ability to sense a mechanical cue requires an intact cytoskeleton and strong physical contact and adhesion to the microenvironment. Importantly, myoblasts also undergo reorientation, alignment and large scale remodeling of the cytoskeleton when they experience mechanical stretch and compression in muscle tissue. It remains unclear if such dramatic changes in cell architecture also inhibit physical contact and adhesion with the tissue microenvironment that are clearly important to myoblast physiology. In this study, we employed interference reflection microscopy to examine changes in the close physical contact of myoblasts with a substrate during induced remodeling of the cytoarchitectu