the pex7-mediated peroxisomal import system is required for fungal development and pathogenicity in magnaporthe oryzae真菌所需的酶pex7-mediated导入系统发展和致病性magnaporthe oryzae.pdfVIP

The PEX7-Mediated Peroxisomal Import System Is Required for Fungal Development and Pathogenicity in Magnaporthe oryzae ¤ Jaeduk Goh, Junhyun Jeon, Kyoung Su Kim , Jongsun Park, Sook-Young Park, Yong-Hwan Lee* Department of Agricultural Biotechnology, Center for Fungal Genetic Resources, Center for Fungal Pathogenesis, Center for Agricultural Biomaterials, Plant Genomics and Breeding Institute, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Korea Abstract In eukaryotes, microbodies called peroxisomes play important roles in cellular activities during the life cycle. Previous studies indicate that peroxisomal functions are important for plant infection in many phytopathogenic fungi, but detailed relationships between fungal pathogenicity and peroxisomal function still remain unclear. Here we report the importance of peroxisomal protein import through PTS2 (Peroxisomal Targeting Signal 2) in fungal development and pathogenicity of Magnaporthe oryzae. Using an Agrobacterium tumefaciens-mediated transformation library, a pathogenicity-defective mutant was isolated from M. oryzae and identified as a T-DNA insert in the PTS2 receptor gene, MoPEX7. Gene disruption of MoPEX7 abolished peroxisomal localization of a thiolase (MoTHL1) containing PTS2, supporting its role in the peroxisomal protein import machinery. DMopex7 showed significantly reduced mycelial growth on media containing short-chain fatty acids as a sole carbon source. DMopex7 produced fewer conidiophores and conidia, but conidial germination was normal. Conidia of DMopex7 were able to develop appressoria, but failed to cause disease in plant cells, except after wound inoculation. Appressoria formed by DMopex7 showed a defect in turgor generation