the rna binding protein sam68 transiently localizes in the chromatoid body of male germ cells and influences expression of select micrornasrna结合蛋白sam68暂时性的本地化拟染色体的男性生殖细胞,影响选择microrna的表达.pdfVIP

The RNA Binding Protein SAM68 Transiently Localizes in the Chromatoid Body of Male Germ Cells and Influences Expression of Select MicroRNAs 1,2 3 2,4 1,5 1 Valeria Messina , Oliver Meikar , Maria Paola Paronetto , Sara Calabretta , Raffaele Geremia , Noora Kotaja3, Claudio Sette1,2* 1 Section of Anatomy, Department of Public Health and Cell Biology, University of Rome ‘‘Tor Vergata’’, Rome, Italy, 2 Laboratory of Neuroembryology, Fondazione Santa Lucia IRCCS, Rome, Italy, 3 Department of Physiology, Institute of Biomedicine, University of Turku, Turku, Finland, 4 Department of Health Sciences, University of Rome ‘‘Foro Italico’’, Rome, Italy, 5 Digestive and Liver Disease Unit, II Medical School, University of Rome ‘‘La Sapienza’’, S. Andrea Hospital, Rome, Italy Abstract The chromatoid body (CB) is a unique structure of male germ cells composed of thin filaments that condense into a perinuclear organelle after meiosis. Due to the presence of proteins involved in different steps of RNA metabolism and of different classes of RNAs, including microRNAs (miRNAs), the CB has been recently suggested to function as an RNA processing centre. Herein, we show that the RNA binding protein SAM68 transiently localizes in the CB, in concomitance with the meiotic divisions of mouse spermatocytes. Precise staging of the seminiferous tubules and co-localization studies with MVH and MILI, two well recognized CB markers, documented that SAM68 transiently associates with the CB in secondary spermatocytes and early round spermatids. Furthermore, although SAM68 co-immunoprecipitated with MVH in secondary spermatocytes, its ablation did not affect the proper localization of MVH in the CB