the role of pas kinase in passing the glucose signal的作用不是在通过葡萄糖激酶的信号.pdfVIP

Sensors 2010, 10, 5668-5682; doi:10.3390/s100605668 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Review The Role of PAS Kinase in PASsing the Glucose Signal Julianne H. Grose 1,* and Jared Rutter 2 1 Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, USA 2 Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112, USA; E-Mail: rutter@ * Author to whom correspondence should be addressed; E-Mail: julianne_grose@; Tel.: +1-801-422-4940; Fax: +1-801-422-0519. Received: 4 February 2010; in revised form: 20 March 2010 / Accepted: 12 May 2010 / Published: 4 June 2010 Abstract: PAS kinase is an evolutionarily conserved nutrient responsive protein kinase that regulates glucose homeostasis. Mammalian PAS kinase is activated by glucose in pancreatic beta cells, and knockout mice are protected from obesity, liver triglyceride accumulation, and insulin resistance when fed a high-fat diet. Yeast PAS kinase is regulated by both carbon source and cell integrity stress and stimulates the partitioning of glucose toward structural carbohydrate biosynthesis. In our current model for PAS kinase regulation, a small molecule metabolite binds the sensory PAS domain and activates the enzyme. Although bona fide PAS kinase substrates are scarce, in vitro substrate searches provide putative targets for exploration. Keywords: PAS kinase; PASKIN; glucose sensor; protei