the response of vocal fold fibroblasts and mesenchymal stromal cells to vibration声襞的反应成纤维细胞和间充质基质细胞振动.pdfVIP

The Response of Vocal Fold Fibroblasts and Mesenchymal Stromal Cells to Vibration 1 2 2 2 1,2 Joel Gaston , Beatriz Quinchia Rios , Rebecca Bartlett , Craig Berchtold , Susan L. Thibeault * 1 Department of Biomedical Engineering, University of Wisconsin Madison, Madison, Wisconsin, United States of America, 2 Division of Otolaryngology Head and Neck Surgery, Department of Surgery, University of Wisconsin Madison, Madison, Wisconsin, United States of America Abstract Illumination of cellular changes caused by mechanical forces present within the laryngeal microenvironment may well guide strategies for tissue engineering the vocal fold lamina propria. The purpose of this study was to compare the response of human vocal fold fibroblasts (hVFF) and bone marrow mesenchymal stem cells (BM-MSC) to vibratory stimulus. In order to study these effects, a bioreactor capable of vibrating two cell seeded substrates was developed. The cell seeded substrates contact each other as a result of the sinusoidal frequency, producing a motion similar to the movement of true vocal folds. Utilizing this bioreactor, hVFF and BM-MSC were subjected to 200 Hz vibration and 20% strain for 8 hours. Immunohistochemistry (Ki-67 and TUNEL) was performed to examine cell proliferation and apoptosis respectively, while semi-quantitative RT-PCR was used to assess extracellular matrix related gene expression. HVFF significantly proliferated (p = 0.011) when subjected to 200 Hz vibration and 20% strain, while BM-MSC did not (p = 1.0). A statistically significant increase in apoptosis of BM-MSC (p = 0.0402) was observed under the experimental conditions; however