the prevalence and regulation of antisense transcripts in schizosaccharomyces pombe粟酒裂殖酵母的患病率和反义转录调节.pdfVIP

The Prevalence and Regulation of Antisense Transcripts in Schizosaccharomyces pombe 1,2. 1,2. 3 1 1,2 4 1 6 Ting Ni , Kang Tu , Zhong Wang , Shen Song , Han Wu , Bin Xie , Kristin C. Scott , Shiv I. Grewal , Yuan Gao4,5, Jun Zhu1,2* 1 Institute for Genome Sciences and Policy, Duke University Medical Center, Durham, North Carolina, United States of America, 2 Genetics and Development Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, United States of America, 3 DOE Joint Genome Institute, Walnut Creek, California, United States of America, 4 Division of Genomics, Epigenomics and Bioinformatics, Lieber Institute for Brain Development, Johns Hopkins University, Baltimore, Maryland, United States of America, 5 Neuroregeneration and Stem Cell Biology Program, Institute of Cell Engineering, Johns Hopkins University, Baltimore, Maryland, United States of America, 6 Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America Abstract A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein- coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense trans