the taxonomic and functional diversity of microbes at a temperate coastal site a ‘multi-omic’ study of seasonal and diel temporal variation微生物的分类和功能多样性在温带沿海站点multi-omic的季节性和一昼夜的时间变异的研究.pdfVIP
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the taxonomic and functional diversity of microbes at a temperate coastal site a ‘multi-omic’ study of seasonal and diel temporal variation微生物的分类和功能多样性在温带沿海站点multi-omic的季节性和一昼夜的时间变异的研究
The Taxonomic and Functional Diversity of Microbes at a
Temperate Coastal Site: A ‘Multi-Omic’ Study of Seasonal
and Diel Temporal Variation
1,2,3 4 1,4 1 1 1
Jack A Gilbert *, Dawn Field , Paul Swift , Simon Thomas , Denise Cummings , Ben Temperton ,
1 5 6 1 1 ¨ 7
Karen Weynberg , Susan Huse , Margaret Hughes , Ian Joint , Paul J. Somerfield , Martin Muhling
1 Plymouth Marine Laboratory, Plymouth, United Kingdom, 2 Argonne National Laboratory, Argonne, Illinois, United States of America, 3 Department of Ecology and
Evolution, University of Chicago, Chicago, Illinois, United States of America, 4 National Environment Research Council (NERC) Centre for Ecology and Hydrology,
Wallingford, Oxfordshire, United Kingdom, 5 Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole,
Massachusetts, United States of America, 6 School of Biological Sciences, University of Liverpool, Liverpool, United Kingdom, 7 TU Bergakademie Freiberg,
¨
IOZ - Interdisciplinary Centre for Ecology, Freiberg, Germany
Abstract
How microbial communities change over time in response to the environment is poorly understood. Previously a six-year
time series of 16S rRNA V6 data from the Western English Channel demonstrated robust seasonal structure within the
bacterial community, with diversity negatively correlated with day-length. Here we determine whether metagenomes and
metatranscriptomes follow similar patterns. We generated 16S rRNA datasets, metagenomes (1.2 GB) and met
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