比色皿使用与鉴别（Use and identification of cuvette）.docVIP

比色皿使用与鉴别（Use and identification of cuvette） Several identification methods: 1, the empty cuvette placed in the scanning instrument inside, you will find that in 200-300nm between the absorption glass cuvette, no absorption is the quartz cuvette, 2, sample room do not put any sample set wavelength 250nm, zero. The colorimetric is placed in the sample channel and the light absorption value is less than 0.07Abs is quartz, and vice versa is glass. 3, the color plate is marked with the letter S is the quartz cuvette, we use is. In addition, it is better to make a contrast in the ultraviolet region. 4, according to Archimedes principle, measuring the density of two cuvette, you can distinguish between quartz or glass cuvette. 5, UV and visible in the cuvette is different, UV required using quartz colorimetric utensil and direction, visible or ultraviolet wavelength of some can use glass, glass to absorb some light in the ultraviolet, against the arrow and down arrow is not measured absorbance consistent. You can use a known solution at a wavelength of maximum absorption test will know, in addition to the light, with an arrow cuvette wool face, the light path and the direction of the arrow direction should be consistent, namely: the arrow points to the detector side. Mark Q and S are quartz quartz cuvette, UV light 200-400nm glass cuvette, visible light 330-1000nm 6, with incandescent lamp irradiation, which transmittance is high, that is glass, quartz inside should be slightly muddy. When a sample has been absorbed in the ultraviolet region, a glass cuvette is used to test the absorption of samples only in visible light. The quartz cuvette does not absorb in the visible and ultraviolet regions, The glass cuvette is absorbed in the ultraviolet region, so it can not be used in the ultraviolet region. Infrared spectrum: 1. Study the structure and chemical bonds of molecules, 2 、 determination of force constants and criterion of molecular symmetry 3. Characterization and iden