MRSA以气溶胶形式致烫伤无毛鼠皮肤感染合并-中国试验动物学报.DOC

周传农修改： 英文摘要— 已修改 正文中的英文书写 — 已顺 图娱表的英文 — 已顺 文献的英文书写 — 已顺 MRSA气溶胶诱导烫伤SKH-1无毛小鼠皮肤感染合并肺炎模型 刘巧玲，朱萍妹1，杨玉琴2，刘芳2，陈颖1，周文江，周光兴1 （1. 复旦大学实验动物科学部，上海 200032；2. 上海市公共卫生临床中心，上海 201508） 【摘要】目的 探讨烫伤条件下，建立MRSA气溶胶致SKH-1无毛小鼠皮肤感染合并肺炎模型的方法及其评价指标。方法 48只SKH-1无毛小鼠，随机分成PBS对照组，单纯感染组和烫伤合并感染组。合并感染组小鼠皮肤烫伤后，MRSA(ST-239)气溶胶呼吸道感染，采用体重、血常规、菌血症率、皮肤和脏器荷菌量、病理组织学变化和分子生物学等指标对模型进行综合评价。结果 与其他两组相比，合并感染组小鼠第三天体重下降明显；白细胞数显著增多；菌血症率达100%；皮肤和肺荷菌量较高，分别达108 CFU/g和106 CFU/g；镜下观察皮肤和肺炎症病变明显；免疫组化结果显示皮肤组织内CD138呈阳性表达；multi-PCR测定感染组织内nuc和mecA基因均为阳性。结论 SKH-1无毛小鼠烫伤再辅以MRSA气雾攻击的方式，可成功建立能够模拟临床上烧烫伤患者继发MRSA肺炎的动物模型，此模型对于抗MRSA药物药效评价研究具有重要临床应用价值。 【关键词】耐甲氧西林金黄色葡萄球菌；气溶胶；烫伤；肺炎；SKH-1无毛小鼠 Establishment of a scald SKH-1 hairless mouse model of skin infection combined with pneumonia induced by MRSA aerosol LIU Qiao-ling1, YANG Yu-qin2, LIU Fang2, CHEN Ying1, ZHOU Wen-jiang2, ZHOU Guang-xing1 (1. Department of Laboratory Animal Science, Fudan University, Shanghai 200032, China; 2. Shanghai Public Health Clinical Center, Shanghai 201508) [Abstract] Objective To establish a scald SKH-1 hairless mouse model of skin and lung infection induced by methicillin-resistant Staphylococcus aureus (MRSA) aerosol and its evaluation criteria. Methods 48 SKH-1 hairless mice were randomly divided into PBS control group, infection group and infection with scald group. After the mouse dorsal skin of the infection with scald group was thermally injured, the mice were challenged with MRSA aerosol (ST-239) through the respiratory tract. The body weight, blood test, bacteremia rate, skin and organs infection of the model mice were measured, and histopathological observation and multi-PCR for the nuc and mecA genes detection were performed. Results Compared with the other two groups, the infection with scald group had evident weight loss on the third day, remarkable increase of WBC count and a bacteraemia rate of 100%. The number of viable S. aureus in the skin and lungs was 108 CFU/g and 106CFU/g, respectively. Inflammatory lesions of the infected tissues were o