基于连锁与关联分析的植物代谢组学研究进展-2015-Luo.pdf

基于连锁与关联分析的植物代谢组学研究进展 刘贤青，董学奎，罗杰* （华中农业大学生命科学技术学院，武汉 430070 ） * 通讯作者：jie.luo@mail.hzau.edu.cn 摘要： 代谢物作为生物体表型的基础能够帮助我们更直观有效地了解生物学过程及其机 理。植物代谢组研究对理解植物代谢途径，改善植物对环境胁迫的应答，提高作物产量 与品质有着非常重要的作用。代谢物的种类和数量在不同品种与组织中存在很大差异； 利用这些差异进行遗传基础的解析将有助于我们深入了解代谢生物学过程。随着代谢组 学分析技术和方法的发展与第二代测序技术的应用，植物代谢组学的研究内容不断地扩 增，代谢遗传机制的研究不断深入。本文概述了植物代谢组学的研究内容，基因型分型、 植物代谢组遗传连锁分析与植物代谢组全基因组关联分析及其应用。 关键词：代谢组学；广泛靶向代谢组学；基因型分型；数量性状座位；全基因组关联 分析；基因功能 Progresses in plant metabolomics using linkage and association mapping strategies LIU Xianqing, DONG Xuekui, LUO Jie* (College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China) Abstract It has been demonstrated that metabolites, as the basis of biological phenotypes, help us to better understand biological processes and provide insights into the biochemical mechanisms of the processes. Research on plant metabolomics plays a vital role in understanding plant metabolic pathways, manipulating plant responses to environmental stresses, improving crop yields and enhancing food quality. Sessile in nature, significant difference could be found in the number and content of metabolites in plants. Dissection of the genetic basis of metabolome thus helps us to extensively understand metabolically biological processes. Moreover, with the advancement of metabolomics and 1 second-generation sequencing, researches in plant metabolomics with its genetic basis are increasing. This review summarizes progresses in plant metabolomics, genotyping, and the understanding of genetic basis of plant metabolome by linkage and association mapping, which can be used as reference for rel