阳启顺毕业论文(初稿1).docVIP

昆 明 学 院 2014 届毕业设计（论文） 设计（论文）题目光照对拟南芥灰霉菌毒素抗性的影响 子课题题目 姓 名 阳启顺 学 号 201014010220 所 属 系 生命科学与技术系 专业年级 10级生物科学2班 指导教师 杨红玉 教授 2014 年 5 月 摘 要 灰霉菌（Botrytis cinerea）能侵染1000多种植物，危害多种粮食和经济作物，造成极大的经济损失。灰霉菌对寄主植物的破坏机制是极其复杂的，致病毒素是其侵染的有力武器之一。灰霉菌产生的毒素可导致植物萎蔫、褪绿和组织坏死等病症，而目前已知的灰霉菌毒素均为小分子有机类物质，对灰霉菌产生的大分子毒素了解不多。因此，分离纯化灰霉菌分泌的大分子毒素，鉴定其致病性对于认识灰霉菌致病的分子机制具有十分重要的意义。 本研究利用离心、过滤和透析等方法对灰霉菌培养液中大分子毒素进行了粗提取，并测定了蛋白浓度。本论文用拟南芥植物为材料，通过针刺渗入法将提取到的灰霉菌的胞外大分子粗提液渗入至拟南芥叶片内，并设置同等浓度的牛血清蛋白、无菌蒸馏水为对照，在光照、黑暗下处理5天，统计观察，结果发现：光照、黑暗下处理的渗有大分子毒素的拟南芥叶片出现病斑，而渗入牛血清蛋白、无菌蒸馏水的对照组没有发现病斑。至于光照对拟南芥灰霉菌毒素抗性影响的程度的估量，还需进一步探究。Abstract Botrytis cinerea can infect more than 1000 kinds of plants, especially harms lots of crops resulting gray mould disease, which cause serious economical losses in agriculture. The mechanisms of destruction host plant of Botrytis cinerea were complex, and the pathogenic toxins is one of powerful weapons when the fungus invades into plants. The toxins which were secreted by B.cinerea can cause a lot of disease symptoms of host, such as wilting, chlorosis, nacrosis etc. Therefore, isolation and purification macromolecular toxins which were secreted by Botrytis cinerea and identification their chemical and pathogenitic property are significant for understanding molecular mechanism of pathogenicity of B. cinerea. In this study, we used the methods of centrifugation, filtration and dialysis for crude extraction of macromolecular toxins from Botrytis cinerea liquid cultural medium. After that, we identified the constituents of the crude extract, and whether the macromolecular toxins were the host-specific toxins. Using Sephadex gel chromatography methods, the crude extract of macromolecular toxins from Botrytis cinerea were separated, and the content of protein were be determinated. The study based on the Arabidopsis thaliana. The extracellular macromolecular crude extrac from B. cinerea were inoculated into Arab