丙泊酚对大鼠急性肾缺血再灌注损伤抗氧化机制探究.docVIP

丙泊酚对大鼠急性肾缺血再灌注损伤抗氧化机制探究 　【摘要】 目的：探讨丙泊酚预处理对急性肾缺血再灌注损伤（acute renal ischemia reperfusion injury, ARIRI）的抗氧化保护作用及其机制。方法：建立大鼠急性肾缺血再灌注损伤模型，健康雄性SD大鼠54只，随机分为3组：假手术组、缺血再灌注(ischemia/reperfusion, I/R)组、丙泊酚预处理组。缺血再灌注前15 min、缺血再灌注后2 h、24 h分别检测血清尿素氮（BUN）、肌酐(Cr)、超氧化物歧化酶（SOD）、丙二醛（MDA）及观察肾组织的病理学变化。结果：丙泊酚预处理组肾组织病理学变化明显轻于缺血再灌注组。与假手术组比较，缺血再灌注组中血清尿素氮、肌酐水平显著增加(Plt;0.05)，而丙泊酚预处理组尿素氮、肌酐水平则低于缺血再灌注组(Plt;0.05)。丙泊酚预处理组血清超氧化物歧化酶和丙二醛水平与缺血再灌注组均有统计学差异(Plt;0.05)。结论：在急性肾缺血再灌注损伤中，丙泊酚能起到对肾脏的保护作用。 【关键词】 丙泊酚；肾脏；缺血再灌注损伤；超氧化物歧化酶；丙二醛 　　Experimental Study about the Protective Effect of Propofol on Acute Renal Ischemia Reperfusion Injury in Rats 　　SONG Yanfang, LIU Jian 　　(The First Affiliated Hospital of XinJiang Medical University, Urumqi， Xinjiang 830054,China) 　　Abstract：Objective To investigate the renal protection and mechanism of propofol in ARIRI rat models. Methods The model of rat’s ARIRI was established. 54 SD rats were randomly divided into 3 groups: false operating group, ischemia reperfusion group and propofol group. BUN, Cr, SOD, and MDA were detected before I/R for 15 minutes and after I/R for 2 hours and 24 hours, and renal histopathology lesions were examined.Results Renal histopathology lesions of propofol preconditioning group decreased significantly after I/R. In I/R group, BUN and Cr serum levels increased significantly after I/R, compared with false operating group(Plt;0.05), while propofol decreased the serum levels of BUN and Cr significantly(Plt;0.05).There was statistic significance of the serum levels of SOD and MDA in Propofol preconditioning group and I/R group(Plt;0.05).Conclusion Propofol preconditioning to kidney could protect the kidney from ARIRI. 　　Key words：Propofol；Kidney；Ischemia reperfusion injury;SOD;MDA 　　缺血再灌注损伤(Ischemic reperfusion injury, IRI)是临床上导致缺血性急性肾功能衰竭的主要原因之一，病死率很高，且存活的患者亦多有不同程度的慢性肾功能损害，因此，如何减轻或避免肾IRI已成为多年来一直倍受关注的问题。丙泊酚作为静脉麻醉药，已证实有良好的抗氧化和抑制细胞因子释放，本研究从肾组织病理学变化、肾功能尿素氮(BUN)、肌酐(Cr)和超氧化物歧化酶(SOD)、丙二醛(MDA)等三个方面探讨丙泊酚预处理对IRI的抗氧化保护作用及其机制。 　　1 材料与方法 　　1.1 材料来源 取54只1