乳腺癌中RUNX3、cyclin D1及p27kip1蛋白表达及其意义.doc

乳腺癌中RUNX3、cyclin D1及p27kip1蛋白表达及其意义 　【摘要】 ［目的］ 探讨乳腺癌组织中RUNX3、cyclin D1和p27kip1基因的表达及其临床意义。［方法］ 采用免疫组织化学SP法检测88例乳腺癌、40例乳腺纤维腺瘤、40例乳腺增生病中RUNX3、cyclin D1和p27kip1的表达。［结果］ （1）RUNX3和p27kip1在乳腺癌中的阳性表达率分别为35.23%和51.14%，明显低于在乳腺纤维腺瘤(85%和82.5%)及乳腺增生病(87.5%和85%)中的表达率（Plt;0.05）。乳腺癌中cyclin D1的表达率（63.63%）明显高于良性病变中表达。（2）RUNX3 和p27kip1蛋白表达与乳腺癌的临床分期、淋巴结转移呈负相关，p27kip1蛋白表达与病理分级呈负相关。cyclin D1蛋白表达与病理分级呈正相关。（3）乳腺癌中RUNX3与p27kip1蛋白的表达呈正相关性（Plt;0.05），与cyclin D1的表达呈负相关性（Pgt;0.05）。［结论］ RUNX3、cyclin D1和p27kip1在乳腺癌的发生发展中起重要作用。检测乳腺癌组织中RUNX3、cyclin D1和p27kip1基因的表达对于评价患者的预后有一定价值。 【关键词】 乳腺肿瘤 Expressions of RUNX3, cyclin D1 and p27kip1 in Breast Cancer and Their Significance Abstract： ［Purpose］ To investigate the expressions of RUNX3, cyclin D1 and p27kip1 in breast cancer and their significance. ［Methods］ Expressions of RUNX3, cyclin D1 and p27kip1 in 88 cases with breast cancer, 40 cases with breast fibroadenoma and 40 cases with mazoplasia were detected by immunohistochemical SP method. ［Results］ (1)The positive expression of RUNX3 and p27kip1 in breast cancer was 35.23% and 51.14% respectively. They were significantly lower than those in breast fibroadenoma (85% and 82.5%) and mazoplasia (87.5% and 85%)(Plt;0.05).The positive expression of cyclin D1 in breast cancer (63.63%) was significantly higher than that in the benign tissues (Plt;0.05). (2)The positive expression of RUNX3 and p27kip1 in breast cancer was negatively correlated to the clinical staging or node metastasis（Plt;0.05）. The expression of p27kip1 was negatively correlated to the histologic grade, but cyclin D1 expression showed positive correlation with histologic grade（Plt;0.05）. (3) The expression of RUNX3 was positively correlated with the expression of p27kip1, and negatively correlated with cyclin D1. ［Conclusion］ RUNX3, cyclin D1 and p27kip1 may play an important role in the carcinogenesis and progression of breast cancer. The detection of RUNX3, cyclin D1 and p27kip1 protein expression has a definite value in evaluating pro