二氮嗪停搏液对缺血再灌注心肌线粒体保护作用.docVIP

二氮嗪停搏液对缺血再灌注心肌线粒体保护作用 　 作者：李寒,张载高,范士志,陈建明,蒋耀光 【摘要】 目的 观察线粒体三磷酸腺苷(ATP)敏感钾离子通道开放剂二氮嗪加入停搏液内对缺血再灌注大鼠心肌线粒体保护作用。方法 50只大鼠随机分为5组，每组10只，取离体心脏，A 组取正常心肌，其余四组置于Langendorff模型。B组停灌30 min, 复灌60 min；其余各组分别灌注不同的停搏液：C组灌注停搏液；D组灌注含二氮嗪的停搏液；E组灌注含二氮嗪停搏液之前10 min给予格列苯脲。各组复灌60 min取标本，电镜下观察线粒体结构改变；提取线粒体，激光共聚焦显微镜扫描检测线粒体膜电位变化；测定线粒体呼吸功能；高效液相法测定心肌ATP含量及能荷(EC)变化。结果 缺血再灌注心肌线粒体结构损伤和功能降低(Plt;0.05)；而二氮嗪处理组相应指标显著改善(Plt;0.01)。结论 含二氮嗪停搏液能够改善缺血再灌注心肌线粒体结构和功能。 【关键词】 心肌缺血再灌注损伤;线粒体;线粒体呼吸控制率;二氮嗪 　　Protective Effect of Cardioplegia with Diazoxide to Myocardial Mitochondrion during Ischemic and Reperfusion in Isolated Working Rat Heart 　　Abstract: OBJECTIVE To evaluate the effects of mitochondrial ATP- sensitive potassium channel opener diazoxide on heart mitochondrion during ischemic and reperfusion in rats. METHODS Fifty rats were divided into 5 groups randomly. Langendorff model was established. Control group (B) underwent 30 minute ischemia and 60minute reperfusion. Group C and D were treated with St.Thomas Ⅱ cardioplegia and diazoxide+St.Thomas Ⅱ cardioplegia before ischemia respectively. And group E was treated with glibenclamide at 10min before Diazoxide+st.Thomas Ⅱ cardioplegia. At the end, mitochondrial changes were detected under the transmission electron microscope(TEM),the respiration function of mitochondrion was determined,ATP,ADP,AMP content of myocardium were determined and energy charge (EC) were calculated. RESULTS Ischemic and reperfusion injury induced the structure damaged and function decrease of myocardial mitochondrial. While in group D(Plt;0.01), the heart was protected effectively by treating with diazoxide. CONCLUSION These results suggest that diazoxide has a protective effect on myocardial mitochondrion during ischemic and reperfusion in rats. 　　Key words： Myocardial ischemic and reperfusion injury; Mitochondrial; Respiration function; Diazoxide 　　缺血再灌注损伤是心脏直视手术后出现心功能不全等并发症的重要原因之一。近年来研究证明, 心肌线粒体三磷酸腺苷敏感性钾通道(MitoKATP)是心肌缺血再灌注损伤的重要治疗靶点。二氮嗪（diazoxide，DZ）是MitoKATP开放剂, 具有对缺血再灌注心肌的保护作用。本研究