云母颗粒对无水乙醇诱导大鼠胃黏膜损伤影响及机理探析.docVIP

云母颗粒对无水乙醇诱导大鼠胃黏膜损伤影响及机理探析 　 作者：钦丹萍 吕海丽 邵国民 任永葆 韩建新 邱添 黄一鸣 【摘要】; 　　［目的］研究云母颗粒对无水乙醇诱导大鼠胃黏膜损伤的保护作用及其机理。［方法］将48只健康雄性SD大鼠随机分为云母颗粒高、中、低剂量组，思密达组，模型对照组，空白对照组6组。分别将云母颗粒和思密达预先灌胃于相应各组大鼠，3h后以1ml/100g无水乙醇灌胃致胃黏膜损伤，45min后下腔静脉取适量血，处死取胃，然后分别检测各组的胃黏膜损伤指数、血清和胃黏膜组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSHPx）含量，并观察病理学变化，以评价胃黏膜损伤程度及药物的作用。［结果］云母各剂量组中SOD、GSHPx含量均显著高于模型对照组，而MDA含量则明显低于模型对照组（Plt;0.05）。［结论］云母颗粒对无水乙醇诱导的大鼠胃黏膜损伤具有预防与保护作用，其保护作用是通过增加大鼠血清和胃黏膜组织中SOD、GSHPx含量并减少MDA的生成来实现的，即云母颗粒具有抗脂质过氧化作用。 【关键词】; 云母颗粒 无水乙醇 损伤指数 损伤程度 超氧化物歧化酶 丙二醛 谷胱甘肽过氧化物酶 　　Abstract：［Objective］ To study the protection and mechanism of mica granules to rats stomach mucosa injury caused by nonwater ethanol.［Method］ Randomly divide 48 healthy SD rats into high，middleand lowdosed groups of mica granules，Simida group，model control group and blank control group as well，i.e.6 groups.After fasting for 24h，separately make stomach perfusion of mica granules and Simida to all rats in advance；after 3h，make stomach perfusion of nonwater ethanol 1ml/100g for stomach mucosa injury；45m later，take proper blood of lower vena cava，sacrifice them for stomach，then respectively test all mucosa injury index，SOD，MDA，and GSHPx content in serum and mucosa，observe pathological changes to evaluate mucosa injury degree and medical function.［Result］ In mica groups，the SOD and GSHPx were markedly higher than that in model control group，but MDA was lower than later（Plt;0.05）.［Conclusion］ Mica granules have prevention and protection to rats gastric mucosa injury caused by nonwater ethanol，which is produced from mica granules’ increasing SOD，GSHPx content and decreasing MDA in serum and gastric mucosa，i.e.mica granules have function of antilipid peroxidation. 　　Key words：mica granules；nonwater ethanol；injury index；injury degree；SOD；MDA；GSHPx ; 　　本实验通过预防性予大鼠灌服云母颗粒，并与思密达作对照，观察云母颗粒对大鼠胃黏膜损伤的防治疗效，同时从血清和胃黏膜组织中SOD、MDA、GSHPx方面探讨其可能的作用机制。 　　1; 实验材料 ; 　　动物模型：健康SD大鼠48只，SPF级，体重200±20g，雄性，购自中科院上海实验动物中心，标准饲料饲养于浙江中医药大学实验动物中心。药物及制备：云母颗粒由浙产绢云母组成，其粒子直径40