化痰泄浊方对脂肪肝模型大鼠胰岛素抵抗及瘦素影响.docVIP

化痰泄浊方对脂肪肝模型大鼠胰岛素抵抗及瘦素影响 　 作者：叶放，赵文霞，苗明三，李建国，张永艳 【关键词】 ,化痰泄浊方 ［摘要］ 目的：探讨化痰泄浊方对大鼠脂肪肝的作用。方法：将SD大鼠随机分为5组：正常对照组、模型组、东宝肝泰组、化痰泄浊方高剂量和低剂量组。用高脂饮食联合四环素腹腔注射法制作大鼠脂肪肝模型，观察大鼠肝脏组织的病理变化，并检测肝功能、血清瘦素、胰岛素抵抗指数，以及肝匀浆甘油三酯（triglyceride, TG）和游离脂肪酸（free fatty acid, FFA）的含量。结果：各用药组的血清瘦素、胰岛素抵抗指数及肝匀浆FFA、TG含量均显著低于模型组（Plt;0.01），且化痰泄浊方组的抗脂肪肝作用显著优于东宝肝泰组（Plt;0.05）。结论：化痰泄浊方治疗脂肪肝的作用机制主要为促进脂质代谢、改善胰岛素及瘦素抵抗。 　　［关键词］ 化痰泄浊方; 脂肪肝; 游离脂肪酸; 胰岛素抵抗; 瘦素抵抗 　　Effects of Huatan Xiezhuo Recipe on insulin resistance and leptin in rats with fatty liver 　　ABSTRACT Objective: To observe the effects of Huatan Xiezhuo Recipe (HTXZR) on fatty liver of rats. Methods:Fortyeight SD rats were randomly divided into five groups: normal control group, untreated group, Dongbao Gantai Tablet (DBGTT)treated group, lowdose HTXZRtreated group and highdose HTXZRtreated group. Fatty liver was induced in the rats by hyperlipid diet and intraperitoneal injection of tetracycline. The pathological changes of liver tissues in rats were observed, and the liver function, serum leptin, insulin resistance index, triglyceride (TG) and free fatty acid (FFA) in the liver were detected. Results: The levels of serum leptin, insulin resistance index, FFA and TG in rats of the three treated groups were lower than those of the untreated group (Plt;0.01), and such effects in the HTXZRtreated groups were more significant than those in the DBGTTtreated group (Plt;0.05). Conclusion:The main action mechanisms of HTXZR in treating fatty liver are probably to promote the lipid metabolism, decrease the leptin and insulin resistance. 　　KEY WORDS Huatan Xiezhuo Recipe; fatty liver; free fatty acid; insulin resistance; leptin resistance 　　当外源性脂肪酸供给增加，游离脂肪酸（free fatty acid, FFA）运输至肝脏过多时，肝细胞内FFA合成甘油三酯（triglyceride, TG）就会增多，从而导致肝内脂肪堆积。既往研究表明，胰岛素抵抗（insulin resistance, IR）和瘦素（leptin）抵抗以及两者之间的相互作用在脂肪肝发病机制中起着重要的作用［1,2］。化痰泄浊法是中医治疗脂肪肝最常用的治法。本实验旨在研究化痰泄浊方（Huatan Xiezhuo Recipe, HTXZR）对高脂饮食联合四环素腹腔注射所致大鼠脂肪肝模型的干预作用及影响环节。 　　1 材料与方法 　　1.1 材料 　　1.1.1 动物 健康雄性SD大鼠48只，体质量（150±10）g，河