基质金属蛋白酶9在宫颈癌中表达及意义.docVIP

基质金属蛋白酶9在宫颈癌中表达及意义 　 作者：姚安梅，高尚风，王晓敏，王英 【摘要】 目的 探讨基质金属蛋白酶9(MMP9)蛋白在宫颈癌中的表达及意义。方法 采用免疫组织化学SP法检测MMP9蛋白在18例正常宫颈上皮、20例宫颈原位癌、55例宫颈浸润癌中的表达。结果 MMP9在宫颈浸润癌中的表达率明显高于正常宫颈组织和宫颈原位癌(Plt;0.05)，而正常宫颈组织和宫颈原位癌组织之间无显著性差异(Pgt;0.05)。MMP9的表达与宫颈鳞癌不同临床分期、肿瘤大小、病理分级无显著性差异(Pgt;0.05)，而在宫颈浸润不同深度间及有无淋巴转移的差异有显著性(Plt;0.05)。结论 MMP9在宫颈浸润癌中呈高表达，MMP9可能通过降解破坏细胞外基质(ECM)和基底膜(BM)而发挥促进宫颈癌发生、发展、浸润转移的作用。 【关键词】 基质金属蛋白酶9(MMP9)；宫颈癌；免疫组化 　　The expression and significance of MMP9 in cervical cancer 　　ABSTRACT: Objective To study the expression and significance of matrix metalloproteinase9 (MMP9) in cervical cancer. Methods The expression of MMP9 protein was tested by using immunohistochemical SP method in 18 cases of normal cervical epithelium, 20 cases of carcinoma in situs of cervix, and 55 cases of invasive cervical cancer. Results The expression of MMP9 protein in invasive cervical cancer was higher than that in normal cervical tissue and carcinoma in situs of cervix (Plt;0.05). There was no significance difference in MMP9 expression between normal cervical tissue and carcinoma in situs of cervix (Pgt;0.05). The expression of MMP9 protein was not related to clinical stages, the size of tumor and pathological grading of invasive cervical cancer (Pgt;0.05), but it was associated with the depth of invasive cervical and lymph node metastases (Plt;0.05). Conclusion The expression of MMP9 was high in cervical cancer. MMP9 may degradate extracelluiar matrix (ECM) and basilemma (BM), thus promoting development and progress, and metastasis of cervical cancer. 　　KEY WORDS: matrix metalloproteinase 9 (MMP9); cervical cancer; immunohistochemistry 　　在肿瘤侵袭、转移过程中，细胞外基质(extracelluiar matrix, ECM)的降解是肿瘤侵蚀正常组织和开始转移的重要途径。ECM的降解依靠蛋白水解酶，这些蛋白水解酶主要可分为4类，基质金属蛋白酶(matrix metalloproteinases, MMPs)是其中较为重要的一类。MMP9是MMPs家族中的重要一员，又称Ⅳ型胶原酶或明胶酶B，在MMPs家族中分子量最大，无活性的前体分子的分子量为92ku，具有酶学活性的蛋白质分子量为82ku，人类MMP9位于染色体20q12-q13，基因全长4506bp。大量研究表明，MMP9在多种恶性肿瘤中呈过度表达，降解破坏ECM中最主要组份Ⅳ型、Ⅴ型胶原和明胶，与肿瘤的浸润转移密切相关[1]。本研究通过免疫组织化学SP法检测MMP9在正常宫颈组织、宫颈