小型猪心肌缺血血瘀证动物模型复制方法.docVIP

小型猪心肌缺血血瘀证动物模型复制方法 　 作者：许文玉, 王伟, 郭淑贞, 刘涛, 刘蕾, 于永新 【摘要】 目的：建立中华小型猪心肌缺血血瘀证的模型。方法：在小型猪左冠状动脉前降支第一间隔支起始部远端放置Ameroid缩窄环，使Ameroid缩窄环内层缓慢膨胀压迫冠状动脉，造成慢性心肌缺血模型。术后，对动物的行为改变、一般情况及血液流变学指标等进行动态观察。结果：手术后4周冠状动脉造影显示心肌缺血小型猪冠状动脉狭窄率大于75％，其行为及一般情况、舌象和血液流变学指标等与术前和同期假手术组相比，差异有统计学意义（Plt;0.05）。结论：术后4周，该模型之证候属于中医血瘀证范畴，而且其发病过程与人类慢性心肌缺血的病理过程相似，适用于心肌缺血血瘀证的研究。 【关键词】 心肌缺血; 血瘀; 模型, 动物; 小型猪 　　Objective: To establish an animal model of myocardial ischemia with blood stasis syndrome in miniswines.Methods: An animal model of myocardial ischemia was established in miniswines by oppressing the coronary artery through the expansion of inner layer of Ameroid constrictor and the Ameroid constrictor was implanted into the distal end of the initial part of the first branch of interventricular septum of ramus descendens anterior arteriae coronariae sinistrae. Dynamic observation of behavior changes, general health status and changes of hemorheological parameters in the miniswines were made after operation.Results: The coronary angiography showed that the stenosis rate in ischemic group was more than 75% four weeks after operation. Compared with before operation and shamoperated group, there were great changes of behavior, general health status, tongue color and hemorheological parameters in ischemic group (Plt;0.05).Conclusion: The animal model of myocardial ischemia with blood stasis syndrome in miniswines was established successfully 4 weeks after operation. The pathological process in the animal model is similar to that in the patients with chronic myocardial ischemia. So this model can be adopted in the research of myocardial ischemia with blood stasis syndrome. 　　Keywords: myocardial ischemia; blood stasis; models, animal; minipigs 证候是中医学认识疾病的主要模式，也是中医诊疗疾病的基石，证候研究一直是中医科研中的重点和难点［13］，合适的证候动物模型是中医药研究获得突破性进展的关键。本研究以冠心病血瘀证作为切入点，构建冠心病（心肌缺血）血瘀证动物模型，以便更方便、更有效地认识冠心病血瘀证的发展规律，进一步指导临床实践。 　　1 材料与方法 　　1.1 实验材料 　　1.1.1 动物与分组 　　动物为中国农业大学育种健康中华小型猪，体质量（25±5）kg，月龄6～10个月，雌雄不限，共27只。随机分为假手术组11只和模型组16只；存活至实验终点且无伤口感染的动物共