生殖支原体表达水平及核苷酸序列变化及女性生殖道炎症相关性分.docVIP

生殖支原体表达水平及核苷酸序列变化及女性生殖道炎症相关性分 　【摘要】 目的：了解女性生殖道中生殖支原体(Mg)的感染情况。方法：用套式聚合酶链反应技术(nPCR)对女性生殖道分泌物211例、组织231例进行Mg 16 SrRNA基因片段检测及核苷酸序列分析。结果：生殖道炎症组（组织24/67例，分泌物59/173例）的检出率均显著高于非炎症组（组织6/164例，分泌物2/38例）P＜0.000 01；在卵巢、输卵管、子宫组织中，宫颈炎（6/30）和宫颈炎性息肉（18/37）的检出率与非炎性组织比较P＜0.05；在各种阴道炎（57/153）中，淋菌性（16/63）、霉菌性（24/52）、滴虫性（13/29）阴道炎检出率与正常人（1/38）比较，差异有显著性，P＜0.000 1；对5例Mg阳性标本的16S rRNA基因片段一级结构序列分析，发现与国际标准株Mg 37同源性达97.8%，有一个T碱基插入。结论：Mg的感染与女性生殖道炎症密切相关。 【关键词】 套式PCR；生殖支原体；生殖道炎症；DNA测序 　　The Prevalence and Signifance of Expression and Nucleotide Sequence of Mycoplasma Gentalium among Female Patients with Reproductive Tract Infectious Diseases 　　LI Lu, YANG Shulan，MOU Qinfeng 　　The Third Affiliated Hospital of Suzhou Univesity，Changzhou, Jiangsu 213003, China Abstract： Objective To find out the prevalence of mycoplasma gentalium(Mg) infection among female reproductive tract infectious diseases. Methods The nested polymerase chain reaction technique and nucleotide sequence analysis targeting the 16SrRNA gene for detecting mycoplasma infection in 211 secretion samples and 231 tissue samples from female reproductive tract infectious disease patients. Results The positive rates of the 16SrRNA gene among patients with reproductive tract infectious diseases are much higher than those among noneinfectous disease patients；The positive rate of 16SrRNA gene among patients with cervicitis and cervices inflammatory polyp is much higher than the other inflammatory reproductive tract deseases. The 16SrRNA gene load among gonorrheal viginitis, trichomonal viginitis and candidly viginitis are much higher than control group respectively. Conclusion Mycoplasma gentalium is closely correlated to the female reproductive tract infectious diseases；The analysis of primary structure of 16 SrRNA revealed that the homology was 97.8% compared with the international standard Mg37 strain, only besides a T insertion. Key words： nPCR； Mycoplasma gentalium； Female reproductive tract infectious diseases； Nucleotide sequence